Method for constructing dairy cow mammary gland differential expression gene regulation and control network under condition of heat stress

Abstract

The invention discloses a method for constructing a dairy cow mammary gland differential expression gene regulation and control network under condition of heat stress. The method comprises the following steps: step one, collecting gland tissue samples of heat stress dairy cow and heat-stress-free dairy cow; step two, enabling Solexa high-throughput sequencing identification to participate in micro RNA (mi RNA) of dairy cow heat stress reaction, and searching differential expression mi RNA; step three, predicting mi RNA target gene with remarkable differential expression; and step four, construction a mi RNA regulation and control network with remarkable differential expression. By the method, the regulation and control effect of mi RNA and the mechanism thereof are disclosed when the dairy cow are in a high-temperature environment, and new test data are provided for regulation and control or weakening of heat stress response of the dairy cow and increasing of milk yield. Meanwhile, the method plays a quite important role in development of heat stress protein feed, regulation and control of an environment for the dairy cow and the like.

Description

Technical field [0001] The invention relates to the technical field of gene regulation networks, in particular to a method for constructing a regulation network of differentially expressed genes in the mammary glands of dairy cows under heat stress conditions. Background technique [0002] With the global warming, the impact of heat stress on animal husbandry has become more and more serious. However, my country has a vast territory, and a large area is affected by heat stress every summer, which leads to metabolic disorders of livestock and poultry, reduced production performance and reproduction performance, and serious economic losses. Heat stress is a self-protection mechanism of the animal body, and the expression of related genes and proteins changes. Studies have shown that heat stress has an important impact on most immune indicators of dairy cows. Most research measures on alleviating heat stress are mainly from the perspective of nutrition. There are few research met...

AI Technical Summary

Problems solved by technology

However, my country has a vast territory, and large areas are affected by heat stress every summer, which leads to metabolic disorders of livestock and poultry, reduced production performance and reproductive performance, and serious economic losses.

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