Application and identification method of biomarkers in identification of effective cold treatment of quarantine fruit flies

Abstract

The invention relates to application of a biological marker in effective cold treatment for identifying a quarantine fruit fly, and also relates to a method for effective cold treatment for identifying a quarantine fruit fly. The method is characterized by comprising the following steps of S1, extracting total metabolite from a to-be-quarantined quarantine fruit fly, and detecting the content of biological marker in the total metabolite; S2, statistically comparing the content of biological marker in the total metabolite in the to-be-quarantined quarantine fruit fly and the contrast data; when the comparison result is obviously different, and the content of biological marker reaches threshold value, judging that the to-be-quarantined quarantine fruit fly is performed with the effective cold treatment. The method has the advantage that the young fly of quarantine fruit fly is quickly screened at high flux by the biological marker, so that the sensitivity and accuracy are high, the judging time in the quarantining treatment observing period is shortened, and the demonstration cost of quarantining treatment experiment is greatly reduced.

Description

technical field [0001] The invention relates to the field of inspection and quarantine, more particularly, the application and identification method of biomarkers in identifying effective cold treatment of quarantine fruit flies. Background technique [0002] With the deepening of the global economic integration process, international trade has become increasingly frequent and diversified, leading to an increase in the frequency of alien pest invasions, posing a huge potential threat to the ecological environment and agricultural production. China is a large fruit import and export country. How to effectively prevent the cross-border spread of harmful organisms, ensure the safety of my country's agriculture, forestry and ecology, and improve the quality of my country's export fruit products has become one of the focuses of my country's entry-exit quarantine departments. [0003] Fruits can carry a variety of dangerous alien pests, especially the destructive quarantine pests ...

AI Technical Summary

Problems solved by technology

At present, the technology of identifying whether insects are subject to quarantine treatment is still in a blank stage, mainly limited to the method of laboratory breeding and observation. It must be cultured in the laboratory before it can be judged whether it has undergone supercooling treatment, or whether the treatment is effective, but it is time-consuming. Longer (generally more than a week), affecting the customs clearance speed of inbound goods

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