Supercharge Your Innovation With Domain-Expert AI Agents!

A method for determining the content and impurities of levocarnitine injection and its application

A technology for injection and impurities, which is applied in the determination of levocarnitine injection content and impurities by hydrophilic interaction chromatography, and in the field of determination of levocarnitine injection content and impurities, which can solve the requirements of low separation limit, column loss, theoretical Reduce the number of plates and other problems, to overcome the effect that is not easy to obtain

Active Publication Date: 2019-09-13
YANTAI UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The United States Pharmacopoeia selects the chromatographic column as an amino-bonded phase chromatographic column. In the method, the demand for the chromatographic column to be equilibrated for up to 3 hours before sample analysis is too strict, which may easily cause column loss, resulting in drift of retention time and decrease in the number of theoretical plates. Shorter column life and lower resolution limit requirements (≥1.0)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for determining the content and impurities of levocarnitine injection and its application
  • A method for determining the content and impurities of levocarnitine injection and its application
  • A method for determining the content and impurities of levocarnitine injection and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] The following examples help to further understand the present invention, but the present invention is not limited to these contents.

[0034] A method for determining the content and impurities of levocarnitine injection by hydrophilic interaction chromatography.

[0035] 1 material preparation

[0036] Shimadzu LC-20A high performance liquid chromatography system (including LC-20AT pump; DGU-20A degasser; SIL-20A autosampler; CTO-10ASvp column thermostat; SPD-20A UV detector; Lab solution chromatography Workstation); WatersXBridge Amide chromatographic column (4.6mm × 250mm, 3.5μm); 1 / 10,000 electronic balance (AR1140, OhausCorp.Brook, NJ, USA); 1 / 100,000 electronic balance (MS105Du type, METTLER TOLEDO); Electronic pH meter (pHMeter PHS-3C, Shanghai Jingke Industrial Co., Ltd.); ultrasonic cleaner (SK250HP, Wuxi Jianyi Experimental Equipment Co., Ltd.).

[0037] The reference substance of levocarnitine was provided by China National Institutes for Food and Drug Cont...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for determining the content and impurities of a levocarnitine injection by hydrophilic interaction chromatography. A result of methodology validation shows that the method is high in degree of separation, has an accurate determination result, can be used for controlling relevant substances of the levocarnitine injection, and provides reference for increase of a quality standard of the levocarnitine injection. Meanwhile, the method can be used for determining the content and impurities of the levocarnitine injection.

Description

technical field [0001] This patent relates to a method for determining the content and impurities of levocarnitine injection, specifically a method for determining the content and impurities of levocarnitine injection by hydrophilic interaction chromatography, which belongs to the field of drug analysis. Background technique [0002] Levocarnitine, whose chemical name is (R)-3-carboxy-2-hydroxy-N,N,N-trimethyl-1-propanamine hydroxide, is an essential natural substance in the energy metabolism of mammals. The main function is to assist cells in lipid metabolism and energy production. [0003] E-4-(trimethylammonium)but-2-enoic acid ethyl ester (referred to as impurity A) is the main impurity and degradation product in levocarnitine, its structure contains a conjugated structure, and the response value is much greater than Levocarnitine, which needs to be quantified separately. [0004] The structural formulas of levocarnitine and its impurity A are as follows, [0005] ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 许丽晓耿银银刘万卉
Owner YANTAI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com