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A gene OsHT1 controlling rice tillering and applications thereof

A gene and rice technology, applied in the control of rice tiller gene OsHT1 ​​and its application field, can solve the problems of reducing the number of tillers and the inability to start axillary meristem normally, and achieve the effect of reducing the plant height

Inactive Publication Date: 2017-06-20
ZHOUKOU NORMAL UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

TAD1 ( Tillering and Dwarf1 ) gene encodes a coactivator protein of the anaphase-promoting complex (APC / C for short), which acts on the upstream of MOC1, can specifically recognize MOC1 and recruit MOC1 to the APC / C complex, resulting in MOC1 is ubiquitinated and degraded in a manner dependent on cell cycle progression, resulting in abnormal initiation of axillary meristems and reduced number of tillers (Xu et al., 2011)

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  • A gene OsHT1 controlling rice tillering and applications thereof
  • A gene OsHT1 controlling rice tillering and applications thereof
  • A gene OsHT1 controlling rice tillering and applications thereof

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Embodiment Construction

[0037] The following invention intends to further OsHT1 The upstream and downstream genes of the gene and the relevant regulatory signaling pathways involved in the analysis were analyzed, and the OsHT1 Whether the gene has a broader yield-enhancing effect. By analyzing the role of this gene in the research on the regulation mechanism of rice tillering and the cultivation of high-yielding varieties, it is hoped to enrich the accumulated data on the molecular mechanism of tillering in rice and other cereal crops, and design and breed high-yielding molecular modules for rice, wheat and other cereal crops through genetic engineering. Provide new genetic resources.

[0038] A rice transgenic line acquisition and phenotype analysis experiment

[0039] 1 Materials and methods

[0040] 1.1 Plant materials and planting methods

[0041] The rice variety tested was the japonica rice variety Zhonghua 11, that is, rice ( Oryza sativa L.) cv. Zhonghua 11, kept in the Key Laborator...

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Abstract

A gene OsHT1 controlling rice tillering is disclosed. The gene is derived from oryza sativa L. cv. Zhonghua 11. The nucleotide sequence of the gene is SEQ ID NO.1, the nucleotide sequence of the open reading frame of the gene is SEQ ID NO.2, and an amino acid sequence encoded by cDNA of the gene is SEQ ID NO.3. Phenotypic analysis shows that after overexpression of the gene OsHT1, the tiller number of rice is increased from about 20-27 to 135-145, plant height is reduced and the setting rate is not changed. The gene OsHT1 is a critical regulating gene for rice tillering, and therefore rice tillering characters can be regulated by utilizing a genetic engineering technology, and the gene has important application value in the field of regulating and controlling plant shape characters on purpose by utilizing a bioengineering technology, and adjusting a plant population structure to achieve a high yield and good quality. The gene can be used for cultivating high-yield varieties of rice, wheat, and other cereal crops.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and relates to an essential gene for plant tillering and application thereof, in particular to a gene OsHT1 ​​for controlling rice tillering and application thereof. Background technique [0002] Rice is one of the main food crops in China and plays an important role in my country's agricultural production. With the rapid increase of population and the continuous reduction of cultivated land area in our country, increasing rice production plays a pivotal role in ensuring our country's food security and promoting the development of national economy. Breeding high-yield rice varieties with ideal plant type is an important aspect of increasing rice yield, and it is also the goal that grain producers have been pursuing. Rice yield is a complex quantitative trait, which mainly depends on the number of effective tillers, the number of grains per panicle and thousand-grain weight. Tillering is a spe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
Inventor 李成伟唐跃辉包欣欣刘坤张菊李晓丽徐克东张怡齐静于德水王健
Owner ZHOUKOU NORMAL UNIV
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