Detection primer for zika virus and applications of detection primer
A Zika virus and primer detection technology, applied in the field of medical testing, can solve problems such as difficulty in primer design, and achieve the effects of accurate and reliable detection results, convenient application, fast and flexible detection
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Embodiment 1
[0051] This embodiment provides a detection primer for Zika virus, the detection primer includes a primer pair, and the base sequences of the primer pair are respectively shown in SEQ ID No.1-2.
[0052]The upstream primer sequences of the detection primers are as follows:
[0053] SEQ ID No.1: 5'-RCTCAAACGAATGGCRGTCAG-3';
[0054] The downstream primer sequences of the detection primers are as follows:
[0055] SEQ ID No. 2: 5'-CAATCAGTTCATCTTGGTGGC-3'.
[0056] The specific method of using the detection primers of Zika virus is as follows:
[0057] 1.1 Take 0.5 μL of the sample to be tested as a template, add 10 μL of 2×Taq Mix, 0.5 μL of each primer pair shown in SEQ ID No.1-2, add ddH 2 O, make up to 20 μL;
[0058] 1.2 The PCR reaction program is: 95°C, pre-denaturation for 5 minutes; 95°C, denaturation for 30s; 54°C, annealing for 30s; 55°C, extension for 30s; 30 cycles; 72°C, 10min.
[0059] 1.3 After the reaction, the reaction product was subjected to agarose gel ...
Embodiment 2
[0063] This embodiment provides a detection kit for Zika virus. The kit contains the detection primers provided in Example 1. The detection primers include primer pairs, and the base sequences of the primer pairs are respectively shown in SEQ ID No.1-2.
[0064] The upstream primer sequences of the detection primers are as follows:
[0065] SEQ ID No.1: 5'-AAGGGTGTTAGGCTTGTCG-3';
[0066] The downstream primer sequences of the detection primers are as follows:
[0067] SEQ ID No. 2: 5'-CAGGCTTAGAAATCAGACG-3'.
[0068] For the method of using the kit provided in this example, refer to the PCR amplification method provided in Example 1.
Embodiment 3
[0070] This embodiment provides a detection kit for Zika virus. The kit contains the detection primers provided in Example 1. The detection primers include primer pairs, and the base sequences of the primer pairs are respectively shown in SEQ ID No.1-2.
[0071] The upstream primer sequences of the detection primers are as follows:
[0072] SEQ ID No.1: 5'-AAGGGTGTTAGGCTTGTCG-3';
[0073] The downstream primer sequences of the detection primers are as follows:
[0074] SEQ ID No. 2: 5'-CAGGCTTAGAAATCAGACG-3'.
[0075] Of course, this kit also includes reagents that can directly perform PCR reactions, including PCR reaction buffer, TaqDNA polymerase, dNTPs, 2×Super Real PreMix, 2×SYBR green I, target fragment standards and Mg 2+ at least one of the
[0076] The usage method of the kit provided in this embodiment is as follows:
[0077] The experimental operation method of fluorescent quantitative PCR is as follows:
[0078] 1.1 Reaction system of fluorescent quantitative P...
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