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Mass spectrometry substrate as well as preparation method and application

A substrate and texture technology, applied in the field of mass spectrometry detection, can solve problems such as complexity, high baseline, and affecting hydrophobic properties

Inactive Publication Date: 2017-06-20
毅新兴业(北京)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In order to overcome the shortcomings of traditional mass spectrometry detection target plate surface without hydrophilic and hydrophobic differences, resulting in poor crystal morphology, low accuracy of mass spectrometry detection sample peaks, low signal-to-noise ratio, high baseline, and high preparation cost and complexity, the droplet contact angle The defect that is too small to affect the hydrophobic performance, the present invention provides an improved substrate for MALDI-TOF detection and has a micro-nano structure. By increasing the difference between the hydrophilicity and hydrophobicity of the target plate on the surface, the sample to be tested is Perfect crystals are formed in the positioning holes, which improves the quality of mass spectrometry detection spectra and the accuracy of identification results

Method used

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  • Mass spectrometry substrate as well as preparation method and application
  • Mass spectrometry substrate as well as preparation method and application
  • Mass spectrometry substrate as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment one, preparation substrate

[0066] (1) Substrate material selection

[0067] It is characterized by tough texture and good flatness; such as stainless steel, diamond, single crystal silicon, quartz crystal, etc.; it is divided into rectangles suitable for MALDI-TOF sample chamber specifications. In order to ensure the accuracy of quality inspection, the division accuracy is less than 10 μm.

[0068] (2) Silanization treatment

[0069] 1. Clean the substrate

[0070] Place the substrates in a beaker, spread out flat without overlapping, rinse the substrates with acetone, and transfer the cleaned substrates to a new beaker.

[0071] Add a certain amount of mixture of methanol and water into the beaker, and ultrasonicate for 30 minutes.

[0072] Transfer the substrate to a new beaker, add a certain amount of chloroform, and sonicate for 30 minutes.

[0073] Remove and dry the substrate.

[0074] 2. Clean the substrate with concentrated acid solution

[00...

Embodiment 2

[0103] Embodiment 2, sample crystallization observation and comparison of target plate

[0104] Take an appropriate amount of the prepared matrix solution and drop them on the two substrates J1 and J2 with contact angles of about 135° and 155°, and the two traditional target plates T1 and T2 respectively; On J1, J2, T1, and T2, after the samples were dried naturally, the crystal morphology was observed under a microscope, see Figure 4 .

[0105] Wherein, the traditional target plate is TO-488 gene detection target plate produced by SHIMADZU.

[0106] Such as Figure 4 As shown, the traditional biological detection target plate T1, T2 crystal form (see Figure 4 ) is irregular in shape, the lines are not smooth, it is in the state of a coffee ring, and the surface is uneven. Due to the hollow space and thick surrounding, when the laser bombards the sample, the sample peak accuracy is poor, the noise is high, and the baseline is high.

[0107] And biological detection subs...

Embodiment 3

[0108] Embodiment three, mass spectrometry detection effect comparison

[0109] Put the substrate J1 covered with the gene sample and the traditional target plate T1 into the MALDI-TOF mass spectrometer for detection.

[0110] parameter settings:

[0111] Turing mode: linear

[0112] Mass Range: 3000-9000

[0113] Max Laser Rep Rate: 10.0

[0114] Power: 90

[0115] Profiles: 40

[0116] Shots: 10

[0117] Compare the detection results of the two target plates.

[0118] Such as Figure 5 As shown, the T1 target plate has a high baseline, few peaks, poor accuracy, and low signal-to-noise ratio; the J1 substrate has a low baseline, more peaks, high accuracy, and a high signal-to-noise ratio.

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Abstract

The invention provides a dedicated substrate carrying a BIOMARK in MALDI-TOF mass spectrometry detection. The substrate comprises a hole-outside hydrophobic region made of a chemical surface modifier, an intra-circle hydrophilic region modified by a special chemical reagent, surface round holes or square holes for titrating a BIOMARK sample, a substrate material having tough texture and good flatness, and a substrate surface having a single-side polished mirror effect. Besides, the substrate can further include a two-dimensional code for identification. The micro/nano structural substrate can effectively enrich the sample, crystal grows uniformly, the mass spectrometry curve sample for BIOMARK biological detection is high in peak accuracy, the signal-to-noise ratio is high, and the base line is low.

Description

technical field [0001] The invention relates to an improved substrate for MALDI-TOF MS and has a micro-nano structure, belonging to the field of mass spectrometry detection. Background technique [0002] MALDI-TOF-MS is the English abbreviation of Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry, that is, Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry, also known as MALDI TOF, which is a new type of soft ionization organic mass spectrometry developed in recent years. By introducing matrix molecules, the molecules to be measured do not generate fragments, which solves the problem of desorption and ionization of non-volatile and thermally unstable biomacromolecules, and is one of the important means for analyzing difficult-to-volatile organic substances. MALDI TOF has been developed by leaps and bounds worldwide, and is widely used in drug development in biotechnology and pharmaceutical companies, biological analysis an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/28G01N33/68B82Y40/00
CPCB81B1/006B81B7/04B81C1/00119B81C1/00206B81C1/00539B82Y40/00G01N1/28G01N1/2813G01N1/34G01N27/62G01N27/64G01N33/6851
Inventor 梁飞马庆伟陈莲莲付书辉梁坤向华
Owner 毅新兴业(北京)科技有限公司
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