Target gene, primer and probes for detecting and identifying cryptococcus and kit

Abstract

The invention discloses a target gene, a primer and probes for detecting and identifying cryptococcus and a kit, which belong to the field of medical mycology. According to the target gene of the cryptococcus, the sequences of the primer for detecting a gene of the cryptococcus is designed, which are shown as SEQ ID No.3 and SEQ ID No. 4 and / or SEQ ID No. 6 and SEQ ID No. 7, wherein the SEQ ID No.3 and the SEQ ID No. 4 correspond to cryptococcus neoformans, the SEQ ID No. 6 and the SEQ ID No. 7 correspond to cryptococcus gattii; and the primer can be used for specific detection of the cryptococcus neoformans and the cryptococcus gattii. Based on the fluorogenic quantitative PCR (polymerase chain reaction) principle, the invention also provides a group of probes for detecting the cryptococcus neoformans and / or the cryptococcus gattii and the detection kit based on the primer and the probes, and the probes and the kit can be used for detecting the cryptococcus neoformans and the cryptococcus gattii that are pathogenic in a same system simultaneously or respectively. The target gene, the primer, the probes and the kit have the advantages of high sensibility, strong specificity, good repeatability and short detection time.

Description

technical field [0001] The invention belongs to the field of medical mycology, and in particular relates to primers, probes and kits for detecting specific target genes of cryptococcus. Background technique [0002] At present, there are many types of Cryptococcus, which are widely distributed in nature. The most closely related to human infection are Cryptococcus neoformans and Cryptococcus gattii. They can cause cryptococcosis in the human lung or central nervous system, and can also cause systemic Disseminated infection. Cryptococcus gattii once caused outbreaks in Vancouver, Canada and the Northwest of the United States. In recent years, infection cases have also been reported in Europe and my country. In my country, Cryptococcus gattii is mainly distributed in Shanghai, Jiangsu, Fujian, Zhejiang, Guangdong, Hainan, and Hebei In other provinces, there are sporadic cases of Cryptococcus neoformans. Cryptococcus neoformans mainly infects immunocompromised patients, while ...

AI Technical Summary

Problems solved by technology

Although ink staining of cerebrospinal fluid can preliminarily observe the morphology of cryptococci, the sensitivity is low; cryptococcus antigen reagents can quickly detect cryptococcus antigens, but cannot distinguish between two main pathogenic cryptococci; cryptococcus culture identification not only takes a long time , and it is difficult for most automated identification instruments to identify Cryptococcus neoformans and Cryptococcus gattii, so that the laboratory can only report Cryptococcus, or may mistakenly report Cryptococcus gattii as Cryptococcus neoformans

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