Method for identification of radix angelicae sinensis, radix levistic officinalis and radix angelicae pubescentis
A technology of angelica and angelica, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems that the methanol extract cannot be distinguished, and cannot be used as a means of distinguishing angelica and angelica, and achieves low equipment requirements, low cost, and simple operation Effect
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Embodiment 1
[0028]Cut Angelica sinensis, Angelica sinensis and Duhuo into uniform powder with a particle size of about 0.5mm, weigh a portion of each, 2.0g each, put each in a 50ml iodine measuring bottle, add 20ml of petroleum ether as a solvent, and ultrasonically extract for 30 minutes. Filtrate, collect the filtrate, dilute to a concentration of about 2 mg / ml, and an absorbance of 0.3000A-2.0000A to obtain the petroleum ether extract of the Chinese medicine to be tested. Utilize the ultraviolet-visible spectrophotometer to carry out the ultraviolet spectrum scanning of range 190.0nm-400nm to the above-mentioned traditional Chinese medicine extract to be tested respectively, obtain as follows figure 1 The ultraviolet spectrum diagram of the shown Angelica sinensis, European angelica and lovage petroleum ether extract, and the maximum absorption peak value of the ultraviolet spectrum line shown in Table 1.
[0029] Depend on figure 1 And table 1 shows: in petroleum ether extract, the u...
Embodiment 2
[0033] With other conditions unchanged, the solvent of the above-mentioned extract was replaced with chloroform to obtain the following: figure 2 The ultraviolet spectrum line diagram of the shown Angelica sinensis, Radix Angelicae sinensis and the chloroform extract of lovage, and the maximum absorption peak value of the ultraviolet spectrum line as shown in Table 2.
[0034] Table 2 Chloroform Extraction Extraction Liquid UV Spectrum Maximum Absorption Peak Value
[0035]
[0036] Depend on figure 2 And table 2 shows: in chloroform extraction solution, the ultraviolet spectrum line figure of lovage extract has absorption valley near 274.0nm, and the ultraviolet spectrum line figure of Chinese angelica and European angelica extract has no absorption valley near 274.0nm.
Embodiment 3
[0038] Other conditions remain unchanged, the solvent of the above-mentioned extract is replaced by absolute ethanol to obtain the following image 3 The ultraviolet spectrum diagrams of the shown Angelica sinensis, European Angelica sinensis and Duhuo absolute ethanol extract, and the maximum absorption peak value of the ultraviolet spectrum line shown in Table 3.
[0039] Table 3 The maximum absorption peak value of ultraviolet spectrum of Angelica sinensis, Angelica sinensis and Duhuo absolute ethanol extract
[0040]
[0041] Depend on image 3 And table 3 shows: in dehydrated ethanol extract, the ultraviolet spectrum line figure of lovage extract has shoulder peak near 250.0nm, has light absorption valley near 271.5nm, and the ultraviolet spectrum of Chinese angelica and European angelica extract The line graph has no shoulder near 250.0 nm and no optical absorption valley near 271.5 nm.
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