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Truffle mycorrhiza seedling synthetic method

A synthesis method and technology of mycorrhizal seedlings are applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., which can solve the problems of low parasitic generation efficiency and low bacterial purity, and achieve the effect of high infection rate.

Inactive Publication Date: 2017-07-07
YUNNAN LYUCHEN BIOTECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the research on the artificial cultivation of truffles has been gradually carried out, but one is that the inoculated truffle strains are all ascomycete homogeneous slurry, and the purity of the strains is low; Under the influence of external bacteria, the infection rate of truffle mycorrhizal seedling synthesis is only less than 60%, and the parasitization rate is low

Method used

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Embodiment Construction

[0011] Below in conjunction with embodiment the present invention is described in detail.

[0012] 1. Cultivation of host plant seedlings:

[0013] (1) Screening and preservation of host plant seeds

[0014] The host plant is any one of Yunnan pine, Huashan pine, chestnut, and Castanopsis japonicus (Castonia chinensis). The seeds of Yunnan pine and Huashan pine are washed with water to remove the empty shell, then dried, and stored in glass jars or gauze bags for later use; Wash the seeds with water to remove mildew, deterioration and unfulfilled seeds, dry the surface moisture, mix the seeds with clean river sand with a humidity of 25-35% in a volume ratio of 1:2, put them in a carton and place them in 4-6 Store in a refrigerated cabinet at ℃ for later use;

[0015] (2) Seed treatment:

[0016] When using Yunnan pine and Huashan pine seeds; soak them in 0.1% potassium permanganate solution for 30 minutes, rinse them with water for 2 to 3 times, and then rinse them with 30%...

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PUM

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Abstract

The invention provides a truffle mycorrhiza seedling synthetic method. The truffle mycorrhiza seedling synthetic method is characterized by comprising the following steps of (1) selecting either pinus yunnanensis, pinus armandi, Chinese chestnut or Castanopsis as a host plant, and culturing the same for 80 to 100 days via sterile substrate under a sterile environment, (2) inoculating truffle cultures in a sterile inoculation room equipped with an air purifier, (3) placing the inoculated host plant to an air purifier-equipped sterile seedling nursing room for 15 days, (4) transplanting the mycorrhiza seedlings to a plastic shed and cultured under the natural environment of the shed, and the transplanting the same to the large field after 5 to 6 months. Liquid truffle mycorrhiza with high purity is utilized; the host plant and the truffle mycorrhiza seedlings are first cultured in the sterile environment, thereby being prevented from infectious microbes; the host plant seedling roots and the surrounding cultures are closely distributed during inoculation; and therefore, truffle mycorrhiza synthetic infectious rate is increased by over 90%, 30% higher than the prior art.

Description

technical field [0001] The invention relates to a method for synthesizing truffle mycorrhizal seedlings. Background technique [0002] Truffles (Truffles) for the cystic fungi, truffles (Tuberales), truffle family (Tuberaceae), truffles (Tuber), is a class of vascular plants that form a symbiotic relationship, with high nutritional value and economic development Valuable underground rare edible fungus, in addition to having high vitamin B 1 , Vitamin B 2 In addition to the unique taste and aroma, the androne components contained in it are unmatched by other edible fungi, especially the truffles in Europe and the United States, which are known as "black diamonds". [0003] In the past, truffles mainly relied on natural growth. With the increase of demand, predatory mining has caused the natural wild production to decline year by year, and it has become an endangered species. In recent years, the research on the artificial cultivation of truffles has been gradually carried ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 寸建清
Owner YUNNAN LYUCHEN BIOTECH DEV CO LTD
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