Tissue culture and rapid propagation method of traditional Chinese medicine cissus assamica

A red-backed silk, tissue culture and rapid propagation technology, applied in the field of plant tissue culture, can solve the problems that there is no red-backed silk tissue culture technology, no patent application for red-backed silk tissue culture and rapid propagation, and achieve low cost and easy operation The effect of strong performance and simple process

Inactive Publication Date: 2017-07-07
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the tissue culture technology of red-backed silk at home and abr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1

[0019] (1) Explant collection: In the field, select the middle and upper parts of the vine plants with robust growth and no obvious disease, and the knotted canes with full sun as explants, and immediately after collection, carry out water retention and moisturizing treatment and bring them back to the laboratory in time .

[0020] (2) Induction culture: when the explants collected in step (1) are collected back to the laboratory, rinse them with tap water overnight, place them on a clean bench, and disinfect them in 75% ethanol solution for 5 seconds. After 5 times of rinsing with sterile water After absorbing the water with sterile filter paper, place it in a 0.1% mercuric solution for 15 minutes of disinfection, rinse it with sterile water for 5 times, absorb the water with sterile filter paper, cut it into 1.5-2.5cm knotted canes and cut them into pieces. Inoculated into the induction medium, placed at 25°C for 45 days in total dark culture, and the for...

Example Embodiment

[0025] Embodiment 2

[0026] (1) Explant collection: In the field, select the middle and upper parts of the vine plants with robust growth and no obvious disease, and the knotted canes with full sun as explants, and immediately after collection, carry out water retention and moisturizing treatment and bring them back to the laboratory in time .

[0027] (2) Induction culture: when the explants collected in step (1) were collected back to the laboratory, rinsed with tap water overnight, placed in a clean bench, and sterilized in 75% ethanol solution for 7 seconds, and rinsed with sterile water for 6 times. After absorbing the water with sterile filter paper, it was placed in 0.1% mercuric solution for 17 minutes of disinfection. After 6 times of rinsing with sterile water, the water was absorbed with sterile filter paper, and then cut into 1.5-2.5cm knotted canes. Inoculated into the induction medium, and placed at 27°C for 47 days in the dark to induce the formation of advent...

Example Embodiment

[0032] Embodiment 3

[0033] (1) Explant collection: In the field, select the middle and upper parts of the vine plants with robust growth and no obvious disease, and the knotted canes with full sun as explants, and immediately after collection, carry out water retention and moisturizing treatment and bring them back to the laboratory in time .

[0034](2) Induction culture: when the explants collected in step (1) are collected back to the laboratory, first rinse them with tap water overnight, place them on a clean bench, and sterilize them in 75% ethanol solution for 10 seconds. After 7 times of rinsing with sterile water After absorbing the water with sterile filter paper, place it in 0.1% mercuric solution for 20 minutes for disinfection, rinse it with sterile water for 7 times, absorb the water with sterile filter paper, cut into 1.5-2.5cm knotted canes and cut them into pieces. Inoculated into the induction medium, placed at 28°C for 50 days in full dark culture, and the...

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PUM

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Abstract

The invention relates to plant tissue culture methods of agricultural biotechnologies, specifically to a tissue culture and rapid propagation method of traditional Chinese medicine cissus assamica. The method includes the following steps in order: acquisition of a cissus assamica explant, induction culture of the explant, subculture, rooting culture and transplantation. An explant induction medium involved in the explant induction culture operation includes: an MS medium, 2.0-4.0mg/L 6-BA, 0.5-1.0mg/L NAA, 20-30g/L sucrose, 3.5-4.0g/L agar, 60-100ml/L coconut milk, and 0.1-0.3g/L activated carbon, and the pH of the explant induction medium is 5.4-5.8. The method provided by the invention selects a cissus assamica stem-segment with node as the explants, carries out induction culture, subculture, rooting culture, transplantation and other steps, reaches an induction rate of 88% or above and a survival rate of 94% or above, and realizes tissue culture and rapid propagation of traditional Chinese medicine cissus assamica, thus realizing the purpose of the invention.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of traditional Chinese medicine red-backed silk. Background technique [0002] Chinese medicine red back silk, that is Cissus assamica (Laws.) Craib., also known as Cissus assamica (Laws.) Craib. It has blood stasis and pain relief effects. It is commonly used in the folk for the treatment of bruises, rheumatic joint pain, carbuncle, swollen poison, snake bites and other diseases, especially poisonous snake bites. Studies have found that red back silk has the effect of antagonizing endothelin, and its ethanol The extract or ethyl acetate extract or the resveratrol isolated therefrom had obvious antagonistic effects on ET-1 at the whole and in vitro levels, and significantly antagonized the induced by ET-1 in isolated rat aortic strips. The contraction is in a dose-dependent manner, and it can anta...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁钧淞莫昭展杨业容韦敏
Owner YULIN NORMAL UNIVERSITY
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