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Human epidermal growth factor receptor Her-2/neu quantitative detection kit and preparation method and application thereof

An epidermal growth factor, quantitative detection technology, applied in the direction of analysis, measurement device, chemiluminescence/bioluminescence, etc. by chemical reaction of materials, can solve the problem of disease-free survival and low overall survival rate, HER2 gene overexpression, endocrine problems such as poor treatment effect, to achieve the effects of long platform stability, improved specificity, and excellent binding efficiency

Inactive Publication Date: 2017-07-07
BEIJING DIACHA BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HER2 gene overexpression occurs in about 30% of breast cancers and is associated with poorer prognosis
Breast cancer patients with HER2 overexpression have rapid disease progression, short chemotherapy remission period, poor endocrine therapy effect, low disease-free survival and overall survival

Method used

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  • Human epidermal growth factor receptor Her-2/neu quantitative detection kit and preparation method and application thereof
  • Human epidermal growth factor receptor Her-2/neu quantitative detection kit and preparation method and application thereof
  • Human epidermal growth factor receptor Her-2/neu quantitative detection kit and preparation method and application thereof

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Embodiment 1

[0069]In the present invention, the capture antibody of the present invention is a monoclonal antibody that can specifically bind to the human epidermal growth factor receptor Her-2 / neu antigen in the human body, and the tracer antibody described in the present invention is a monoclonal antibody that can specifically bind to the human epidermal growth factor receptor Her-2 / neu specific binding monoclonal antibody. In addition to the specific binding of the capture antibody and the tracer antibody to the human epidermal growth factor receptor Her-2 / neu, they can form a "sandwich" sandwich structure with the antigen when paired.

[0070] 1. The human epidermal growth factor receptor Her-2 / neu antigen used in this example was purchased from Wuxi Aorui Dongyuan Biotechnology Co., Ltd. The human epidermal growth factor receptor Her-2 / neu capture antibody used in this example and The tracer antibody was purchased from Wuxi Aorui Dongyuan Biotechnology Co., Ltd. The formula of vario...

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Abstract

The invention provides a human epidermal growth factor receptor Her-2 / neu quantitative detection kit and a preparation method and application thereof. The kit is prepared through the magnetic particle chemiluminescent immunoassay, wherein a reconstructed Her-2 / neu antigen serves as a standard substance antigen and is diluted to a protein buffer component containing a non-ionic surfactant, the dispersity of the antigen is improved, autoagglutination is prevented, and the activity is maintained; besides, the Her-2 / neu protein antigen and magnetic particles are coupled, and the stability of a magnetic separation component is good by means of the optimized curing method and the blocking method; besides, by means of combined use of a multi-component immune complex and Roche active interference-eliminating proteins MAK33, the heterophil antibody interference in tumor marker sandwich method detection can be solved, and specificity of a finished product is improved. The kit is excellent in property, low in cost and long in validity period.

Description

technical field [0001] The invention belongs to the field of medical and biological immunological detection reagents, in particular to a human epidermal growth factor receptor Her-2 / neu quantitative detection kit and its preparation method and application, more specifically, to a magnetic particle separation Kit for quantitative detection of tumor marker human epidermal growth factor receptor Her-2 / neu by chemiluminescence method and preparation method thereof, and method for quantitative detection of tumor marker human epidermal growth factor receptor Her-2 / neu by using the kit . Background technique [0002] The human epidermal growth factor receptor Her-2 / neu gene is a proto-oncogene originally discovered in the DNA of chemically induced rat neuroblastoma and glioblastoma, also known as c-erB2, It consists of 922 adenines, 1,382 cytosines, 1,346 guanines and 880 thymines. It is located on human chromosome 17q21 and encodes a transmembrane glycoprotein (p185) with a relat...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/531G01N33/535G01N33/553G01N21/76
CPCG01N21/76G01N33/531G01N33/535G01N33/553G01N33/577
Inventor 刘劲
Owner BEIJING DIACHA BIO ENG
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