Hybridization method for raising pollination success rate of distant hybridization peanuts

Abstract

The invention discloses a hybridization method for raising pollination success rate of distant hybridization peanuts, comprising the following steps: (1) acquiring male flower pollens of a male parent during the full-bloom stage of peanuts; (2) gathering pollens of a female parent during the flowering stage of peanuts, and carrying out hyperthermic inactivation by the use of a thermostat water bath pan; (3) mixing the male flower pollens of the male parent acquired in the step (1) and the inactivated pollens of the female parent prepared in the step (2), and preparing a pollen suspension by the use of a cane sugar + boric acid mixed solution; (4) uniformly smearing the pollen suspension onto pistil stigma of peanut female parent; (5) greatly controlling the insect pests until fruit ripening; and (6) carrying out progeny selection, comparison between different strains and selection of excellent strains into evaluation nursery, and carrying out two-year multi-point yield and quality qualification testing so as to obtain a new peanut variety. The hyperthermic inactivated female parent's pollens have strong fusion activity. By using the pollens as an inducer for pollen fusion, compatibility of the pollen suspension is enhanced so as to raise the success rate of peanut hybridization.

Description

technical field [0001] The invention belongs to the technical field of peanut breeding, and in particular relates to a hybridization method for improving the pollination success rate of distant hybrid peanuts. Background technique [0002] In the artificial cross breeding of distant hybrid peanuts, the pollen of each male parent should be attached to the stigma of the pistil. Because the affinity between the pollen of the male parent and the stigma of the pistil is not strong, the heterogeneous pollen cannot germinate normally on the stigma, and the pollen tube cannot be formed after the pollen germinates. Entering the stigma, the pollen tube grows slowly or is too short, resulting in a low hybridization success rate of distant hybrid peanuts. Contents of the invention [0003] The object of the present invention is to provide a hybridization method for improving the success rate of pollination of distant hybrid peanuts in view of the deficiencies of the above-mentioned pr...

AI Technical Summary

Problems solved by technology

[0002] In the artificial cross breeding of distant hybrid peanuts, the pollen of each male parent should be attached to the stigma of the pistil. Because the affinity between the pollen of the male parent and the stigma of the pistil is not strong, the heterogeneous pollen cannot germinate normally on the stigma, and the pollen tube cannot be formed after the pollen germinates. Entering the stigma, the pollen tube grows slowly or is too short, resulting in a low hybridization success rate of distant hybrid peanuts