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A method of tissue culture and rapid propagation of Paulownia combined with rooting outside the bottle

A tissue culture rapid propagation and external rooting technology, applied in the field of plant tissue culture, can solve the problems of long cycle, high cost, complicated process, etc., and achieve the effects of easy transportation, reasonable formula, and avoiding damage to the root system

Active Publication Date: 2019-01-18
广东新桐林业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Paulownia tissue culture rapid propagation method combined with out-of-bottle rooting technology for the traditional tissue culture rapid propagation technology with complex process, long cycle and high cost, and to establish a highly efficient tissue culture rapid propagation system, Thereby increasing the propagation speed of seedlings and reducing the cultivation cost of Paulownia tissue culture seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Select the semi-lignified branches of Paulownia that are healthy and free of diseases and insect pests, use conventional disinfectant to disinfect the surface of the branches, and then trim the stems with a length of 3.0 cm and at least one axillary bud as explants, and gently scrub the surface of the stems with soft gauze , soaked in 1% washing powder solution for 5 minutes, rinsed with running water for 5 minutes; then placed on the ultra-clean workbench, soaked the explants in 75% ethanol solution for 30 seconds, washed with sterile water for 5~ 6 times; then soak the explants in 0.1% mercuric chloride solution for disinfection for 5-10 minutes, and wash them with sterile water for 5-6 times; keep stirring during each soaking period.

[0032] Cut off 1-2 mm at both ends of the treated explants, and inoculate them on the induction medium to induce initial buds; the raw material components and mass content of the induction medium are: improved MS + 6-BA 2. 0 mg / L + IBA...

Embodiment 2

[0039] Select semi-lignified branches of Paulownia that grow robustly and are free from diseases and insect pests. Use conventional disinfectant to sterilize the surface of the branches, and then trim them to a length of 3.5-4.5 cm with at least one axillary bud as explants. Gently scrub the surface of the stem segment, soak in 1% washing powder solution for 5 minutes, rinse with running water for 5 minutes; then place it on the ultra-clean workbench, soak the explant in 75% ethanol solution for 30 seconds, and use Wash the explants with sterile water 5-6 times; then soak the explants in a 0.1% mercuric chloride solution for 5-10 minutes for disinfection, and wash them with sterile water 5-6 times; keep stirring during each soaking period.

[0040] Cut off 1-2 mm at both ends of the treated explants, and inoculate them on the induction medium to induce initial buds; the raw material components and mass content of the induction medium are: improved MS + 6-BA 3.0 mg / L + IBA 1.5 ...

Embodiment 3

[0047] Select the semi-lignified branches of Paulownia that grow robustly and are free from diseases and insect pests. Use conventional disinfectant to disinfect the surface of the branches, and then trim the stems with a length of 4.5-5.0 cm and at least one axillary bud as explants. Gently scrub the stems with soft gauze The surface of the section was soaked in 1% washing powder solution for 5 minutes, rinsed with running water for 5 minutes; then placed on an ultra-clean workbench, soaked in an ethanol solution with a volume concentration of 75% for 30 seconds, and washed with sterile water 5-6 times; then soak the explants in 0.1% mercuric chloride solution for disinfection for 5-10 minutes, wash with sterile water 5-6 times; keep stirring during each soaking period.

[0048] Cut off 1-2 mm at both ends of the treated explants, and inoculate them on the induction medium to induce initial buds; the raw material components and mass content of the induction medium are: improve...

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Abstract

The invention discloses a paulownia tissue culture and rapid propagation method combining an ex-vitro rooting technology. The method comprises the steps of explant treatment, initial bud induction culture, subculture bud proliferation culture, nursery substrate preparation, subculture bud transplantation and post-transplantation management, and comprises the concrete steps of selecting paulownia semi-lignified branches which grow robustly without plant diseases and insect pests; clipping the branches into stem segments as an explant; sterilizing the explant, and inoculating the explant into an initial bud induction medium so as to obtain an initial bud; inoculating the initial bud into a subculture medium so as to obtain a tissue culture subculture bud, cutting the subculture bud which grows robustly and has the height being larger than or equal to 2.5cm, and transplanting the subculture bud into a nursery substrate; after carrying out post-transplantation management, obtaining paulownia seedlings. The method provided by the invention is simple and feasible, the process is simplified, the seedling period is shortened, the survival period from initial bud induction to seedling transplantation is minimized to 45 days, the speed of seedling culture is accelerated, the production efficiency is improved, the cost is reduced, and the method has better economic benefit, social benefit and ecological benefit.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a paulownia tissue culture rapid propagation method combined with an out-of-bottle rooting technology. Background technique [0002] Paulownia is a collective name for deciduous trees belonging to the genus Paulownia of the family Scrophulariaceae. It is suitable for a wide range of growth and has been planted in 23 provinces, municipalities, and autonomous regions in my country. It is one of the tree species with the longest history of artificial cultivation in China. Paulownia wood has the characteristics of light weight, strong fire resistance, not easy to split, not easy to deform, and moisture insulation, corrosion resistance, easy drying and good acoustic performance. , strong anti-pollution and air purification ability, is an important greening and beautifying tree species in cities and suburbs; Paulownia leaves, flowers, and wood have medicinal effects such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G7/06C05G3/00C05G3/02C05G3/60
CPCA01G7/06A01G24/00A01G31/00A01H4/001A01H4/005C05D9/00C05G3/00C05G3/60C05F11/02
Inventor 邹明洋陈政璋
Owner 广东新桐林业科技有限公司
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