GRNA (guide Ribonucleic Acid) sequence capable of effectively knocking out CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) of HTLV-1 (Human T-cell Leukemia Virus type 1) virus genome

A virus genome and sequence technology, applied in the field of gRNA sequences, can solve the problems of secondary infection, ineffective removal of HTLV-1, etc., and achieve the effect of inhibiting proliferation
CN106957844AInactive Publication Date: 2017-07-18HUAQIAO UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAQIAO UNIVERSITY
Publication Date
2017-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a gRNA (guide Ribonucleic Acid) target sequence capable of effectively knocking out CRISPR / Cas9 (Clustered regularly interspaced short palindromic repeats / CRISPR associated protein 9) of an HTLV-1 (Human T-cell Leukemia Virus type 1) virus genome and application of the gRNA target sequence. The gRNA target sequence comprises a first RNA forward sequence, a second RNA forward sequence, a first RNA reverse sequence and a second RNA reverse sequence, wherein the first RNA forward sequence and the first RNA reverse sequence are complementary with each other and comprise sequences shown as SEQ ID NO 01 and SEQ ID NO 02 respectively; the second RNA forward sequence and the second RNA reverse sequence are complementary with each other and comprise sequences shown as SEQ ID NO 03 and SEQ ID NO 04 respectively. The gRNA sequence disclosed by the invention can be used for effectively knocking out the HTLV-1 virus genome and can be used for effectively inhibiting proliferation of ATL (Adult T-cell Leukemia) cells and occurrence of tumors.
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Description

Technical field

[0001] The invention belongs to the field of biotechnology, and specifically relates to a CRISPR / Cas9 gRNA sequence that can effectively knock out the HTLV-1 virus genome. Background technique

[0002] Adult T-cell leukemia (adult T-cell leukemia, ATL) is a malignant lymphoid system proliferative disease caused by human T cell lymphotropic virus type I (Human T cell lymphotropic virus, HTLV-1) infection. Currently, there is no treatment or prognosis for it The ideal plan seriously threatens human health. The high incidence of HTLV-1 infection in my country is concentrated in the coastal areas of Fujian and Taiwan. HTLV-1 is the first retrovirus found to be related to human diseases, with an incubation period of up to 40 years. When it infects host cells, it can randomly integrate its genome into host cell chromosomes. Existing treatment methods are very It is difficult to remove the latent virus in the host.

[0003] About 20 million people in the world are infecte...

Claims

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