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Method used for detecting anammox

A technology of anaerobic ammonium oxidation and bacteria, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of time-consuming, high cost, cumbersome procedures, etc., and achieve the effect of simple operation

Inactive Publication Date: 2017-07-21
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Aiming at the disadvantages of cumbersome procedures, high cost and long time-consuming in the current detection process of anammox bacteria, the present invention proposes a convenient method for detecting anammox bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] The samples containing anammox bacteria in this example were taken from the anammox sludge cultivated and domesticated by Guilin University of Technology. The specific detection steps are as follows:

[0014] (1) Wash the collected anammox sludge with clean water.

[0015] (2) The cleaned anammox sludge was stained with Sudan red.

[0016] (3) Wash the stained anammox sludge to remove excess Sudan red.

[0017] (4) Add commercially available anti-fluorescence quenching mounting solution to the cleaned anammox sludge for anti-fading treatment.

[0018] (5) Place the anammox sludge after the anti-fading treatment in a fluorescence spectrophotometer to analyze and detect the fluorescence in the 500-570nm band, and qualitatively and quantitatively detect the anammox bacteria in the sample according to the obtained fluorescence value.

[0019] Comparing the fluorescence values ​​obtained in this example with the analysis results obtained by advanced molecular biology means...

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Abstract

The invention discloses a method used for detecting anammox. The method comprises following steps: samples containing anammox are collected; the samples containing anammox are subjected to pretreatment; after pretreatment, the samples containing anammox are subjected to dyeing; after dyeing, the samples containing anammox are subjected to washing so as to remove excess colouring agent; after washing, the samples containing anammox are subjected to anti-fading treatment; after anti-fading treatment, the samples containing anammox are subjected to analysis detection using a fluorospectro photometer or a fluorescence microscope, and qualitative and quantitative determination of anammox in the samples are carried out based on the fluorescence values at certain wavelengths. Operation of the method is simple; and low-cost rapid accurate analysis detection of anammox is realized.

Description

technical field [0001] The invention belongs to the field of bacteria detection, in particular to a method for detecting anammox bacteria. Background technique [0002] The anammox process is a low-cost, high-efficiency denitrification process that has emerged in recent years. Its denitrification mechanism is based on the life metabolism of anammox bacteria to remove ammonia nitrogen and nitrite nitrogen. In the anammox process, anammox bacteria (also known as "anammox bacteria") is one of the key microorganisms for the stable operation of the process. Therefore, the detection of anammox bacteria is the key to ensure the operation of the anammox process One of the necessary measures. At present, researchers mostly carry out qualitative and quantitative analysis of anammox bacteria by means of advanced molecular biology. However, this method has disadvantages such as cumbersome experimental process, high cost, and long time consumption. Based on this, the present invention p...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 张文杰金樾王敦球
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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