Method for inducing wheat multiple shoots by use of wheat mature embryo germinated buds
A mature embryo and bud induction technology, applied in the field of crop genetics and breeding, can solve the problems of difficult induction, poor stability, and low induction efficiency, and achieve the effects of high efficiency, improved genetic transformation efficiency of wheat, and stable and feasible technical routes
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Embodiment 1
[0032] Such as figure 1 As shown, using the wheat seeds harvested half a year in 2015 of the common wheat Xiaoyan 22 variety as material, this embodiment provides a method for inducing clustered buds of wheat by using mature wheat embryos to germinate, and the specific steps are as follows:
[0033] Step 1. Peeling mature embryos of wheat variety Xiaoyan 2 and inoculating them on medium Ⅰ to induce germination of mature wheat embryos
[0034] Select Xiaoyan 22 seeds that have been harvested for half a year and have full grains, wash them twice with sterile water, disinfect them with 70% alcohol for 1 minute under sterile conditions, soak the seeds with 0.1% mercury liter for 15 minutes, and wash them 4 times with sterile water After invading in warm water at 25°C overnight, after the seeds of Xiaoyan 22 are completely imbibed, take out the seeds and rinse them with 70% ethanol for 30 seconds, then wash them with 0.1% HgCl 2 Disinfect for 10 minutes, wash with aseptic water fo...
Embodiment 2
[0048] This example uses the wheat seeds of the Xinong 1376 strain harvested half a year in 2015 as materials, and provides a method for inducing clustered buds of wheat by using mature wheat embryos to germinate. The specific steps are the same as in Example 1.
[0049] figure 2 It shows the growth of adventitious buds from mature embryos of Xinong 1376 variety. It can be seen that there are 7 adventitious buds, and similar results have been obtained in repeated experiments. It can be seen that this method has high stability in inducing clustered buds of wheat.
Embodiment 3
[0051] This example uses the wheat seeds of the Xinong 183 strain harvested for half a year in 2015 as materials, and provides a method for inducing clustered buds of wheat by using the germination bodies of mature wheat embryos. The specific steps are the same as in Example 1.
[0052] image 3 It shows the growth of adventitious buds from mature embryos of Xinong 183 variety. It can be seen that there are 7 adventitious buds, and similar results have been obtained in repeated experiments. It can be seen that the method of inducing clustered buds of wheat has high stability.
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