A method for rapid propagation of walnut tissue culture to eliminate yellowing of tissue culture seedlings

Abstract

The invention discloses a walnut tissue culture and rapid propagation method for eliminating yellowing of tissue culture seedlings. The walnut tissue culture and rapid propagation method includes working procedures of propagation material selection, explant treatment, initial bud induction and proliferation culture, and particularly includes acquiring tender walnut shoots which robustly grow in current years and are free of plant diseases and insect pests; pruning branches to obtain stem segments to be used as explants; carrying out sterilization and disinfection treatment on the explants, then inoculating the explants in initial bud induction media to induce initial buds to germinate; inoculating the initial buds into proliferation media to obtain tissue culture subculture buds without yellowing phenomena. The tender walnut shoots are used as propagation materials. The walnut tissue culture and rapid propagation method has the advantages that the problems of deterioration of proliferation coefficients due to chlorosis and yellowing during subculture culture in walnut tissue cultivation, rapid propagation and seedling growing procedures and severe influence on normal differentiation and growth of walnut tissue culture seedlings are explored on the basis of acquisition of aseptic seedlings and can be solved by the aid of the walnut tissue culture and rapid propagation method, the yellowing phenomena of the walnut tissue culture seedlings can be eliminated, the tissue culture seedlings can be normally differentiated and can normally grow, the propagation coefficients can be increased, genetic characters can be stabilized, and accordingly requirements of industrial seedling growing standards can be met by the walnut tissue culture seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a walnut tissue culture rapid propagation method for eliminating yellowing of tissue culture seedlings. Background technique [0002] Walnut (Juglans regia L.) Known as walnut in ancient times, it belongs to Juglandaceae and Juglans. It is native to southeastern Europe and western Asia. my country is one of the origins of walnut. Because walnut is an important nut and woody oil tree species, it has high economic value and ranks first in the world's four major dry fruits (walnut, almond, cashew nut, hazelnut), and is famous in the world for its high economic, ecological and social benefits Widely cultivated in various places. In the process of long-term cultivation and artificial selection of walnuts, many excellent walnut varieties with high yield, high quality and good disease resistance have been formed. In order to maintain the excellent characteristics of these v...

AI Technical Summary

Problems solved by technology

Due to the superiority of plant tissue culture technology, Chinese and foreign scholars began to study the tissue rapid propagation technology of walnut from the late 1960s and made some progress. However, some key technologies in the subculture of walnut tissue culture have not been developed for many years. Can be well solved, such as walnuts are very prone to chlorosis and yellowing during subculture, and the yellowing rate is as high as more than 95%, which seriously affects the normal differentiation and growth of walnut tissue culture seedlings, resulting in a greatly reduced proliferation coefficient, making Industrialization of walnut tissue culture seedlings has been difficult to achieve