A class of skeleton flavanols in eagle tea and its separation and preparation method
A separation method and technology of eagle tea, applied in the direction of organic chemistry, etc., can solve the problems of inability to separate fat-soluble components, difficult to obtain trace components, large sample loss, etc., achieving good controllability and reproducibility, low cost, The effect of reducing sample loss
Active Publication Date: 2019-04-12
ZUNYI MEDICAL UNIVERSITY
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See: Wang Jing, Lu Weili, Zhang Yilong, et al. Research on the chemical constituents of eagle tea [J]. Journal of Anhui Medical University, 2014, 49(4): 479-483. The disadvantage is that the ethyl acetate part has not been systematically separated, and the obtained Also polar flavonoids and their glycosides
[0004] The above methods focus on the use of chromatographic techniques for the separation of highly polar flavonoids, which cannot be separated to obtain fat-soluble components. The separation methods used have a large loss of samples, and it is not easy to obtain trace components.
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Embodiment 1
[0024] The preparation method of skeleton flavanol compound of the present invention comprises the following steps:
[0025] 1) Take 1.6kg of the leaves of Eagle Tea tree derived from Leopard Pi camphora, dry and pulverize them, extract 3 times with ethanol with a volume fraction of 95% under reflux, and concentrate under reduced pressure to obtain 210g of ethanol extract;
[0026] 2) After dispersing the ethanol extract in step 1) in water, extract with petroleum ether, ethyl acetate and n-butanol successively for 3 times, and concentrate under reduced pressure to obtain extracts of various parts;
[0027] 3) Combine the petroleum ether and ethyl acetate parts in step 2) on a TLC plate and perform silica gel column chromatography, use petroleum ether-ethyl acetate at a volume ratio of 100:0, 9:1, 8:2, 6:4 , 0:100 gradient elution, combined with TLC detection method, divided into 7 components according to the polarity of flavanols, Fr.1~Fr.7, each of the following components i...
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The invention discloses a preparation method of new skeleton structured flavanols separated and purified from Litsea coreana. A traditional extraction technology is combined with a modern chromatographic separation technology to separate and purify the new skeleton structured flavanols from the Litsea coreana. The method concretely includes the following steps: immersing extraction, solvent extraction, normal phase column chromatographic gradient elution, and semi-preparative high performance liquid chromatographic separation. The new skeleton compounds obtained by separation and purification are named as catechin-[6,7-bc]-4-(undec-10-en-1-yl)-dihydro-2(3H)-pyranone. The method for extracting and separating the new component from the Litsea coreana allows the new skeleton flavanol components to be extracted and separated from the Litsea coreana, and provides a material basis for the further pharmacology and medical efficacy study of the Litsea coreana.
Description
technical field [0001] The invention relates to separation and extraction technology in the field of chemistry, in particular to a new type of flavanol skeleton in Guizhou eagle tea and a method for its separation and preparation. Background technique [0002] Eagle tea is originally produced in Dalou Mountain, and it is still in wild and semi-wild state. It is a plant substitute tea that has been drunk by various ethnic groups in southern my country for a long time, especially in Sichuan, Chongqing and Zunyi, Guizhou. Studies have shown that eagle tea has anti-oxidation, hypoglycemic, lipid-lowering, anti-inflammatory, antibacterial, antiviral and other effects, and has great development and application prospects, which is in line with the theme of general health research advocated by the state. [0003] Most of the compounds in eagle tea derived from Litsea coreana Lévl.var.lanuginosa (Migo) Yang et P.H.Huang are flavonoids, because they contain more phenolic hydroxyl grou...
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IPC IPC(8): C07D493/04
CPCC07D493/04
Inventor 肖世基成蕾张茂生何芋歧
Owner ZUNYI MEDICAL UNIVERSITY