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Carbon dot for fluorescent image imaging, preparation method and application

A carbon dot and imaging technology, applied in the field of biological carbon dot preparation, can solve the problems of non-environmentally friendly raw materials, high cost, and difficulty in obtaining them, and achieve the effects of low raw material cost, good dispersibility, and good biocompatibility

Inactive Publication Date: 2017-08-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a carbon dot for fluorescent imaging, its preparation method and application, which solves the problems of high cost, non-environmental protection or difficult to obtain raw materials in the existing carbon dot preparation method

Method used

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  • Carbon dot for fluorescent image imaging, preparation method and application
  • Carbon dot for fluorescent image imaging, preparation method and application
  • Carbon dot for fluorescent image imaging, preparation method and application

Examples

Experimental program
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Embodiment 1

[0033] A carbon dot for fluorescence imaging, specifically prepared according to the following steps:

[0034] Step 1, mixing 2g of 4-hydroxyethylpiperazineethanesulfonic acid, 1g of hydrogen peroxide and 8g of ultrapure water to obtain a raw material mixture solution;

[0035] Step 2, placing 5 g of the raw material mixture solution in a high-pressure reactor, the pressure of the high-pressure reactor is less than or equal to 3 MPa, and heating at 180°C for 18 hours to obtain a reaction mixture solution;

[0036] Step 3, put 5ml of the reaction mixture solution in a dialysis bag, wherein the molecular weight cut-off of the dialysis bag is 500Da, add 400ml of ultrapure water, dialyze for 24h, and then concentrate the solution outside the dialysis bag by rotary evaporation to remove water to obtain carbon point crude oily liquid;

[0037] Step 4, crudely dissolve 1g of carbon dots in 1g of methanol to obtain a crude solution; then use methanol as a developer to purify the crud...

Embodiment 2

[0040] A carbon dot for fluorescence imaging, specifically prepared according to the following steps:

[0041] Step 1, mixing 4g of 4-hydroxyethylpiperazineethanesulfonic acid, 2g of hydrogen peroxide and 16g of ultrapure water to obtain a raw material mixture solution;

[0042] Step 2, placing 5 g of the raw material mixture solution in a high-pressure reactor, the pressure of the high-pressure reactor is less than or equal to 3 MPa, and heating at 180°C for 20 hours to obtain a reaction mixture solution;

[0043] Step 3, put 5ml of the reaction mixture solution in a dialysis bag, wherein the molecular weight cut-off of the dialysis bag is 500Da, add 400ml of ultrapure water, dialyze for 26h, and then concentrate the solution outside the dialysis bag by rotary evaporation to remove water to obtain carbon point crude oily liquid;

[0044] Step 4, crudely dissolve 1g of carbon dots in 1mg of methanol to obtain a crude solution; then use methanol as a developer to purify the cr...

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Abstract

The invention discloses a preparation method of a carbon dot for fluorescent image imaging. The method includes: mixing 4-hydroxyethylpiperazine ethane sulfonic acid, hydrogen peroxide and ultrapure water in a mass ratio of 2:1:8 evenly to obtain a raw material mixture solution; carrying out reaction on the raw material mixture solution under high temperature and high pressure conditions to obtain a reaction mixture solution; subjecting the reaction mixture solution to dialysis with a dialysis bag, and conducting rotary evaporation concentration on the dialyzed solution, and performing silica gel column purification treatment, thus obtaining a high purity carbon dot. The method provided by the invention utilizes materials common in life, has the advantages of low raw material cost, simple synthesis process and good biocompatibility, and can achieve large-scale preparation. The carbon dot prepared by the method has good dispersibility in water, is suitable for cell fluorescence image imaging, and has very broad application prospect in biological science and clinical medicine.

Description

technical field [0001] The invention belongs to the technical field of biological carbon dot preparation, and in particular relates to a carbon dot for fluorescence image imaging, a preparation method and an application. Background technique [0002] As a new nanomaterial, carbon dots are widely used in biological sciences and clinical medicine, especially for their fluorescent properties and their ability to enter cells through endocytosis. Carbon dots play a particularly important role in cell imaging. protrude. Carbon dots have excitation dependence within a certain range, that is, their emission wavelength redshifts with the redshift of the excitation wavelength, which is also one of the conditions for judging whether they are carbon dots. Compared with other fluorescent nanoparticles, carbon dots have the characteristics of low toxicity, stable fluorescence performance and good biodescriptive properties, which means that they are more suitable for application in biolog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C01P2004/64C01P2006/60C09K11/65G01N21/6486
Inventor 陈志俊王晓亮孙康博
Owner JILIN UNIV