Method for cryopreservation and resuscitation of decidua parietalis tissue and isolation and culture of mesenchymal stem cells

A technology for stromal stem cells, isolation and culture, applied in the field of cell biology, can solve the problems of difficulty in obtaining maternal-derived mesenchymal stem cells, consuming manpower, material resources and time costs, avoiding toxic effects and serum-derived contamination, and saving cells. The effect of passage time and strong proliferation ability

Inactive Publication Date: 2017-08-08
GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0007] However, the methods and technologies for isolating stem cells from decidual placental tissue are not yet fully mature, and it is difficult to obtain pure maternal-de...

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  • Method for cryopreservation and resuscitation of decidua parietalis tissue and isolation and culture of mesenchymal stem cells
  • Method for cryopreservation and resuscitation of decidua parietalis tissue and isolation and culture of mesenchymal stem cells
  • Method for cryopreservation and resuscitation of decidua parietalis tissue and isolation and culture of mesenchymal stem cells

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Embodiment Construction

[0035] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0036] Method for cryopreservation of parietal decidua tissue

[0037](1) Collection and pretreatment of placental tissue

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Abstract

The invention relates to a method for cryopreservation and resuscitation of decidua parietalis tissue and isolation and culture of mesenchymal stem cells. The method includes the steps of: (1) pretreatment of placenta tissue; (2) separation and treatment of placenta decidua parietalis tissue; (3) cryopreservation of placenta decidua parietalis tissue; (4) resuscitation of placenta decidua parietalis tissue; (5) separation of mesenchymal stem cells from placenta decidua parietalis tissue; and (6) subculture of decidua parietalis stem cells. The method provided by the invention cuts the stripped decidua parietalis tissue into pieces for direct cryopreservation. Treatment of each tissue and culture of isolated cells both need certain time and staff consumption. Therefore, the practice of cryopreservation of decidua parietalis tissue and resuscitation for stem cell isolation when needed relatively more conforms to cost-effectiveness.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to a method for freezing, resuscitating and isolating and culturing mesenchymal stem cells of parietal decidual tissue. Background technique [0002] With the rise of regenerative medicine, cell therapy is being continuously developed. Mesenchymal stem cells have become a research hotspot in the field of cell therapy because of their wide range of sources, easy isolation and culture, and strong proliferation and multi-directional differentiation capabilities. [0003] Mesenchymal Stem Cells (MSCs) originate from the mesoderm and are a type of adult stem cells. Because of the advantages of wide source, easy acquisition, strong expansion ability, low immunogenicity, non-tumor, ability to homing, and no medical ethics disputes, it has become the main cell of choice in regenerative medicine and cell therapy. It has been found that this kind of cells can differentiate into a vari...

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Application Information

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IPC IPC(8): C12N5/0735A01N1/02
CPCA01N1/0221C12N5/0037C12N5/0605C12N5/0606C12N2500/25C12N2500/32C12N2500/38C12N2500/90C12N2501/11C12N2501/115C12N2501/125C12N2501/135C12N2501/39C12N2501/998
Inventor 王进辉李东升许峻荣杨阳陈智聪钟翠婷于莉
Owner GUANGDONG VITALIFE BIOTECHNOLOGY CO LTD
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