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Method for extracting theanine from tea leaves

A technology for theanine and tea, which is applied in pharmaceutical formulations, preparations for skin care, separation/purification of carboxylic acid amides, etc., can solve the problems of potential safety hazards in chemical synthesis methods, high cost of microbial fermentation methods, and weak practical applicability. , to achieve the effect of low production cost, speed up filtration and relieve pressure

Inactive Publication Date: 2017-08-11
唐雪平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used theanine preparation methods include chemical synthesis, microbial fermentation, and tea extraction. Among them, the chemical synthesis has potential safety hazards, and by-products will be produced during the synthesis process, causing environmental pollution; the cost of microbial fermentation is relatively high. High, weak practical applicability; and the tea extraction method often used in the prior art has complicated steps, many types of equipment, and also has the problem of high extraction cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: a kind of method extracting theanine from tealeaves, comprises the steps:

[0023] (1) Extraction: crush the tea leaves, then place them in an extractor, add water at 45-60°C to soak for 2 hours, and pass through a 50-mesh sieve to obtain a preliminary filtrate; the volume ratio of tea leaves to water is 1:15 ;

[0024] (2) Cross-flow filtration: inject the preliminary filtrate in step (1) into the cross-flow filtration system for cross-flow filtration, the reflux ratio is 40%, and obtain the secondary filtrate;

[0025] (3) Microfiltration: microfiltration is performed on the secondary filtrate, and the pore size of the microfiltration is 0.5um;

[0026] (4) Adsorption: Put the secondary filtrate on the chromatographic column for adsorption and separation, and collect the effluent; wherein, the filler in the chromatographic column is polyamide resin;

[0027] (5) Concentration: use a reverse osmosis membrane with a molecular weight cut-off of 100 to con...

Embodiment 2

[0029] Embodiment 2: a kind of method extracting theanine from tealeaves, comprises the steps:

[0030] (1) Extraction: crush the tea leaves, then place them in an extractor, add water at 45-60°C to soak for 6 hours, and pass through a 50-mesh sieve to obtain a preliminary filtrate; the volume ratio of tea leaves to water is 1:8 ;

[0031] (2) Cross-flow filtration: inject the preliminary filtrate in step (1) into the cross-flow filtration system for cross-flow filtration, the reflux ratio is 80%, and obtain the secondary filtrate;

[0032] (3) Microfiltration: microfiltration is performed on the secondary filtrate, and the pore size of the microfiltration is 0.2um;

[0033] (4) Adsorption: Put the secondary filtrate on the chromatographic column for adsorption and separation, and collect the effluent; wherein, the filler in the chromatographic column is a macroporous adsorption resin;

[0034] (5) Concentration: use a reverse osmosis membrane with a molecular weight cut-off...

Embodiment 3

[0036] Embodiment 3: a kind of method extracting theanine from tealeaves, comprises the steps:

[0037] (1) Extraction: crush the tea leaves, then place them in an extractor, add water at 45-60°C to soak for 4 hours, and pass through a 50-mesh sieve to obtain a preliminary filtrate; the volume ratio of tea leaves to water is 1:10 ;

[0038] (2) Cross-flow filtration: inject the preliminary filtrate in step (1) into the cross-flow filtration system for cross-flow filtration, the reflux ratio is 60%, and obtain the secondary filtrate;

[0039] (3) Microfiltration: microfiltration is performed on the secondary filtrate, and the pore size of the microfiltration is 0.4um;

[0040] (4) Adsorption: Put the secondary filtrate on the chromatographic column for adsorption and separation, and collect the effluent; wherein, the filler in the chromatographic column is macroporous adsorption resin or polyamide resin;

[0041] (5) Concentration: use a reverse osmosis membrane with a molecu...

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PUM

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Abstract

The invention belongs to the technical field of theanine extraction and production, and specifically relates to a method for extracting theanine from tea leaves; including extraction, cross-flow filtration, microfiltration, adsorption, concentration, drying and other steps; the tea leaves provided by the invention The method for extracting theanine in the invention can shorten the process steps, reduce the extraction cost, and have high product yield.

Description

technical field [0001] The invention belongs to the technical field of theanine extraction and production, and in particular relates to a method for extracting theanine from tea leaves. Background technique [0002] Along with the continuous development of tealeaves, its health care function is more and more favored by people. Among them, theanine is a unique free acid in tea, which accounts for 1-2% in dry tea. It is the main component of tea that promotes body fluids and quenches thirst. products have broad application prospects. Currently commonly used theanine preparation methods include chemical synthesis, microbial fermentation, and tea extraction. Among them, the chemical synthesis has potential safety hazards, and by-products will be produced during the synthesis process, causing environmental pollution; the cost of microbial fermentation is relatively high. High, practical applicability is weak; and the tea extraction method that often adopts in the prior art has ...

Claims

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Application Information

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IPC IPC(8): C07C237/06C07C231/24A61K8/44A61Q19/10A61Q5/02
CPCC07C231/24A61K8/44A61Q5/02A61Q19/10C07C237/06
Inventor 苗欣雨
Owner 唐雪平
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