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Pestalotipsis vismiae CAMT66351 and applications thereof

A technology of CAMT66351, Pseudomonas simulans, applied in the field of microorganisms, can solve rare problems, achieve wide application value, and achieve the effect of large-scale fermentation and production

Active Publication Date: 2017-08-11
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports about Cordyceps fungus, but there are few articles about Cordyceps mole crickets, especially the isolation of asexual strains from it

Method used

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  • Pestalotipsis vismiae CAMT66351 and applications thereof
  • Pestalotipsis vismiae CAMT66351 and applications thereof
  • Pestalotipsis vismiae CAMT66351 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 strain isolation, screening and identification

[0024] 1. Sample processing

[0025] The mole cricket and Cordyceps samples were packed in nylon-polyethylene bags (7×10cm transparent food vacuum bags), and stored at low temperature until use. First rinse with tap water for 15 minutes, then rinse with sterile normal saline for 3 times, then disinfect with 75% ethanol, and finally rinse with sterile normal saline. Use tweezers and scissors that have been dried and sterilized at a high temperature on a sterile operating table to separate the worm body from the claws and cut them into pieces; weigh 5.0 g of the worm body sample and 5.0 g of the claw sample, and place them in a cone filled with 45 mL of normal saline. In the shaped bottle, make a 1:10 sample solution, that is, the insect body sample solution and the paw sample solution.

[0026] Prepare a sterile test tube containing 9 mL of normal saline, draw 1 mL of 1:10 sample solution with a 1 mL sterile pi...

Embodiment 2

[0054] The fermentation culture of embodiment 2 bacterial strains

[0055] The purified and morphologically stable strains are subjected to liquid fermentation culture. Potato liquid medium and rice liquid medium are used for cultivation. First inoculate the pure strains into the test tube liquid culture medium and cultivate for 7 days, then pour the culture medium and colonies in the whole test tube into the liquid Erlenmeyer flask culture medium, and cultivate them in a constant temperature incubator at 20°C to obtain high-yield bacteria silk, which is convenient for later determination of nutrient content.

[0056] The growth effect of endophytic fungal isolates of Cordyceps fungus in the three mediums in order from good to poor is: rice medium, potato medium, PDA medium, and a more impressive culture medium can be designed according to the nutrient content of the rice medium formula. Among them, the growth effect of rice medium and potato medium was similar, and there wa...

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Abstract

The invention belongs to the field of the microbial technology, and discloses a pestalotipsis fungus and applications of the pestalotipsis fungus. Through the analysis on the separation and character variation of endophytic fungi of cordyceps sinensis, culture, isolation and purification are carried out on the mole cricket insect body and claws by adopting different culture media, then the pestalotipsis fungus is separated and screened, and the fungus is preserved in the Guangdong Microbiological Culture Collection Center on Dec 26, 2016, and is assigned with the accession number of GDMCC No: 60135. The fungus is in good growth conditions under the conditions that the rice culture medium is adopted, the temperature is 20 DEG C and no illumination is available, nucleoside with high content can be produced, the discovery of the fungus can realize the abundant fermentation and production of nucleoside in vitro, and thus the fungus has the wide application value.

Description

technical field [0001] The present invention relates to the technical field of microbes, and more specifically, relates to a Wesmera spp. CAMT66351 and its application. Background technique [0002] Mole cricket Cordyceps is a complex of bacteria and insects whose growth mode is similar to that of Cordyceps sinensis, that is, the fungus infects the mole cricket body, decomposes the tissues of the body of the mole cricket and uses its nutrients to obtain energy sources, and then forms the mole cricket Cordyceps sinensis. Although mole crickets and Cordyceps are extremely rare, the effective active substances contained in Cordyceps have the effect of medical care. Wild Cordyceps grows in high altitude areas. Cordyceps contains 15 kinds of trace elements such as amino acids, nucleosides, sugars, alcohols, vitamins and organic acids. "Compendium of Materia Medica Supplements" mentioned that Cordyceps "works the same as ginseng". In addition, its pharmacological functions includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P19/40C12P19/38C12R1/645
CPCC12P19/385C12P19/40C12N1/145C12R2001/645
Inventor 王雅玲叶日英孙力军
Owner GUANGDONG OCEAN UNIVERSITY
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