Pestalotipsis vismiae CAMT66351 and applications thereof
A technology of CAMT66351, Pseudomonas simulans, applied in the field of microorganisms, can solve rare problems, achieve wide application value, and achieve the effect of large-scale fermentation and production
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Embodiment 1
[0023] Example 1 strain isolation, screening and identification
[0024] 1. Sample processing
[0025] The mole cricket and Cordyceps samples were packed in nylon-polyethylene bags (7×10cm transparent food vacuum bags), and stored at low temperature until use. First rinse with tap water for 15 minutes, then rinse with sterile normal saline for 3 times, then disinfect with 75% ethanol, and finally rinse with sterile normal saline. Use tweezers and scissors that have been dried and sterilized at a high temperature on a sterile operating table to separate the worm body from the claws and cut them into pieces; weigh 5.0 g of the worm body sample and 5.0 g of the claw sample, and place them in a cone filled with 45 mL of normal saline. In the shaped bottle, make a 1:10 sample solution, that is, the insect body sample solution and the paw sample solution.
[0026] Prepare a sterile test tube containing 9 mL of normal saline, draw 1 mL of 1:10 sample solution with a 1 mL sterile pi...
Embodiment 2
[0054] The fermentation culture of embodiment 2 bacterial strains
[0055] The purified and morphologically stable strains are subjected to liquid fermentation culture. Potato liquid medium and rice liquid medium are used for cultivation. First inoculate the pure strains into the test tube liquid culture medium and cultivate for 7 days, then pour the culture medium and colonies in the whole test tube into the liquid Erlenmeyer flask culture medium, and cultivate them in a constant temperature incubator at 20°C to obtain high-yield bacteria silk, which is convenient for later determination of nutrient content.
[0056] The growth effect of endophytic fungal isolates of Cordyceps fungus in the three mediums in order from good to poor is: rice medium, potato medium, PDA medium, and a more impressive culture medium can be designed according to the nutrient content of the rice medium formula. Among them, the growth effect of rice medium and potato medium was similar, and there wa...
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