Method suitable for establishing low-serum Vero cell line

Abstract

The invention relates to the technical field of cell culture, in particular to a method suitable for establishing a low-serum Vero cell line. Vero cells are cultured with a gradient serum concentration culture solution; the series of defects of Vero cells in a high-serum environment, such as high culturing cos and damage of cells by serum components, are overcome; the method is suitable for establishing the low-serum Vero cell line, the cell line adaptive to low-serum environments is cultured, and the method has the advantages of time saving, labor saving, reduced hematogenous pathogenic contamination, lower cost and the like. The method is already successfully applied to the culture of Vero cells and is successfully applied to the study in terms of vaccine production and acquisition of high-quality high-titer viral culture solutions; the Vero cell line suitable for low serum has good morphology and high proliferation speed and is directly applicable to commercial development.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a method for establishing a low-serum Vero cell line. Background technique [0002] Serum is a substance added when culturing cells in vitro. It is a very complex mixture separated from plasma by removing fibrinogen. The serum used for cell culture is mainly bovine serum. Serum is rich in nutrients necessary for cell growth and plays an important role in the cell culture process. [0003] In order to cultivate cells with complete morphology and rapid proliferation, appropriate medium is needed. The commonly used medium is to add calf serum to provide nutritional factors and simulate a stable growth environment in vitro, but the composition of serum is complex, and many components are still unknown It is not clear, therefore, that there are many disadvantages and potential risks associated with the extensive use of serum in cell culture. ① There may be blood-borne pathogeni...

AI Technical Summary

Problems solved by technology

② There are hundreds of ingredients in serum, and current science and technology are still difficult to explain clearly the properties and mechanism of action of all the ingredients

④ There are quality differences in different batches of serum, making it difficult to guarantee the quality of vaccines

⑤ The serum extraction process is complicated, the source is difficult, and the price is very expensive, which brings difficulties to large-scale production

[0004] In summary, when the cells are used in the production of biological products such as vaccines, the high serum level will damage the cells to a certain extent, making it difficult to guarantee the quality of biological products. In addition, the high cost of serum brings difficulties to production, so Cells need to be acclimated to low serum conditions for culture and application