Unlock instant, AI-driven research and patent intelligence for your innovation.

Real-time fluorescent rpa kit, test strip rpa kit and application thereof for rapid detection of porcine pseudorabies virus

A porcine pseudorabies virus and detection kit technology is applied in the field of porcine pseudorabies virus detection kits, test strip RPA kits, and achieves the effects of increasing specificity, being easy to carry, and having real and reliable detection results.

Active Publication Date: 2020-06-16
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention is aimed at the gD gene of porcine pseudorabies virus, establishes and evaluates the RPA (real-time RPA) detection kit based on fluorescent probe and the LFS RPA detection kit based on test strips to realize rapid detection of porcine pseudorabies virus Purpose, as far as we know, there is no such kit at home and abroad to detect porcine pseudorabies virus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Real-time fluorescent rpa kit, test strip rpa kit and application thereof for rapid detection of porcine pseudorabies virus
  • Real-time fluorescent rpa kit, test strip rpa kit and application thereof for rapid detection of porcine pseudorabies virus
  • Real-time fluorescent rpa kit, test strip rpa kit and application thereof for rapid detection of porcine pseudorabies virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The establishment of the real-time fluorescent RPA (Real-time RPA) detection kit and detection method of rapid detection porcine pseudorabies virus of embodiment 1

[0055] 1. Design and preparation of primers and probe sequences

[0056]The inventors of the present invention obtained from GenBank AY217094.1, Y14834.1, KM189914.3, KT253413.1, KR051961.1, KP257591.1, KP315913.1, KM061380.1, KP098534.1, KP259831.1 , KC981239.1, KF017335.1 and AY196984.1 gD gene homologous sequences were compared and analyzed, and the conserved region of porcine pseudorabies virus gD gene was determined, and primers and probes were designed for this region, in order to detect as much as possible Possibly more porcine pseudorabies virus. All primers and probes were synthesized by Sangon Biotech (Shanghai, China). The present invention designed and synthesized three pairs of upstream primers, three pairs of downstream primers and one probe sequence respectively, as shown in Table 1 below. ...

Embodiment 2

[0090] The establishment of the test strip RPA (LFS RPA) detection kit and detection method of embodiment 2 rapid detection porcine pseudorabies virus

[0091] 1. Design and preparation of primers and probe sequences

[0092] The inventors of the present invention obtained from GenBank AY217094.1, Y14834.1, KM189914.3, KT253413.1, KR051961.1, KP257591.1, KP315913.1, KM061380.1, KP098534.1, KP259831.1 , KC981239.1, KF017335.1 and AY196984.1 gD gene homologous sequences were compared and analyzed, and the conserved region of porcine pseudorabies virus gD gene was determined, and primers and probes were designed for this region, in order to detect as much as possible Possibly more porcine pseudorabies virus. All primers and probes were synthesized by Sangon Biotech (Shanghai, China). The present invention designed and synthesized three pairs of upstream primers, three pairs of downstream primers and one probe sequence respectively, as shown in Table 1 below.

[0093] Table 1, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a real-time fluorescence RPA kit for rapid detection of the porcine pseudorabies virus and application thereof, and further discloses a test strip RPA kit for rapid detection of the porcine pseudorabies virus and application thereof. The two kits comprise primers as shown in SEQ ID NO.1 and SEQ ID NO.2 respectively and respective probes. Though aiming at the same target sequence, modified bases and detection platforms of the primers and the probes are different. Experiments prove that the two kits have the same sensitivity which is 102 copies per reaction, and both kits can only specifically detect the porcine pseudorabies virus. When the two kits are used for detecting a porcine pseudorabies virus suspected sample, results show that the detection results of both kits accord with qPCR completely. Therefore, both kits can be used for detecting the porcine pseudorabies virus quickly, efficiently and sensitively, and an effective technical means is provided for differential diagnosis of the porcine pseudorabies virus.

Description

technical field [0001] The invention relates to a kit for detecting porcine pseudorabies virus and its application, in particular to a real-time fluorescent RPA kit and test strip RPA kit for rapidly detecting porcine pseudorabies virus based on RPA reaction, and also relates to two The invention relates to the rapid detection of porcine pseudorabies virus, and the invention belongs to the field of preventive veterinary inspection. Background technique [0002] Thirty years have passed since the birth of PCR technology. From classic PCR, real-time quantitative PCR to the current digital PCR, this technology is constantly changing but has never faded out of our field of vision. However, this technology requires special and expensive thermal cycle instruments and skilled operators, which is time-consuming and labor-intensive, and it is difficult to be used in on-site diagnosis and places with poor experimental conditions. In the laboratories of developing countries, due to t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12R1/93
CPCC12Q1/6844C12Q1/705C12Q2521/507C12Q2561/113C12Q2563/107C12Q2565/625
Inventor 张志东杨洋秦晓东张伟李志勇
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com