Electrophoresis tank device for isoelectric focusing electrophoresis

Abstract

The invention provides an electrophoresis tank device for isoelectric focusing electrophoresis. By adding an electrode rotation axis to an original device and arranging an electrode end internal through hole of the electrode rotation axis at an electrode end, the horizontal swing of the electrode end during the operation of the device is effectively avoided; by arranging a plurality of flexible assemblies in an electrode end contact system, the up and down vibration during the operation of the device is effectively buffered and the effective contact between the electrode end and the surface of an adhesive tape in a focusing groove is ensured; through the buckle clamping connection of the electrode end contact system and the anti-buckle clamping connection between the electrode end contact system and a working table of an electrophoresis tank, a device service life problem caused by contact friction between the electrode end and a power supply is reduced and an adhesive tape distortion phenomenon caused by the vibration of the focusing groove during installation is also reduced. The innovative device ensures the stability of electrophoresis current to a certain extent and enhances the effect of material focus purification.

Description

technical field [0001] The invention relates to the field of biotechnology separation and detection equipment, in particular to an electrophoresis tank device for isoelectric focusing electrophoresis. Background technique [0002] At present, two-dimensional electrophoresis (Two-dimensional electrophoresis, 2DE) is a kind of isoelectric focusing (IEF) electrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SDS-PAGE) combined with higher resolution protein electrophoresis detection technology (Cai, Z., Chiu, J.F., He, Q.Y., 2005, Current Proteomics, 4(2), 259-268); in the electrophoresis of IEF , isoelectric focusing electrophoresis makes the separation of proteins form a linear arrangement and can complete the purpose of sample detection and analysis, and in some cases can quantify protein samples. [0003] At present, isoelectric focusing experiments are usually carried out on gel strips cont...

AI Technical Summary

Problems solved by technology

Although the contact area between the electrode end and the isoelectric focusing electrophoresis strip has been greatly increased, and the poor electrical contact has been improved, the easy swing of the electrode end will cause the uneven electric field of the isoelectric focusing electrophoresis strip; in the experimental operation, The tedious assembly of accessories and the cleaning of the device after the experiment have caused new troubles; this not only greatly increases the workload of the researchers and consumes a lot of time, but also leads to misunderstandings due to poor contact of the contact device.

Images