Composition for reducing inhibition of nucleic acid amplification
A technology of isothermal nucleic acid amplification and composition, applied in the direction of DNA preparation, recombinant DNA technology, biochemical equipment and methods, etc.
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Embodiment approach A
[0103] Embodiment A is a composition comprising:
[0104] Organic iron chelating reagent;
[0105] ferric iron;
[0106] A nonionic surfactant at a concentration greater than or equal to 0.005% (mass / volume); and
[0107] wherein the pH of the composition is from about 8.45 to 8.85;
[0108] Wherein the organoiron chelating reagent has a ratio greater than 10 relative to ferric iron 4.2 The first affinity constant and relative to magnesium is less than 10 3.8 The second affinity constant, wherein the first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20°C.
[0109] Embodiment B is the composition of embodiment A, further comprising water, wherein the nonionic surfactant is present in the composition at a concentration of greater than or equal to 0.005% (mass / volume).
[0110] Embodiment C is the composition of embodiment A or embodiment B, wherein the organoiron chelating agent comprises a plurality of carboxylate gro...
Embodiment approach AP
[0152] Embodiment AP is a kit comprising:
[0153] ferric iron;
[0154] Organoiron chelating reagents; and
[0155] Nonionic surfactants;
[0156] Wherein the organoiron chelating reagent has relative to ferric iron greater than or equal to 10 4.2 The first affinity constant and relative to magnesium is less than 10 3.8 The second affinity constant, wherein the first affinity constant and the second affinity constant are determined in deionized water at pH 8.45 and 20°C.
[0157] Embodiment AQ is the kit according to embodiment AP, wherein the organoiron chelating reagent comprises a plurality of carboxylate groups.
[0158] Embodiment AR is the kit according to embodiment AP, wherein the organoiron chelating reagent is selected from the group consisting of: ethylene glycol tetraacetic acid; N,N,N',N'-tetrakis(2-pyridylmethyl)ethane -1,2-diamine; 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; N-(2-hydroxyethyl)ethylenediamine-N , N',N'-triacetic acid; and the...
Embodiment approach BC
[0169] Embodiment BC is the kit according to any one of embodiments AP to BB, wherein the kit further comprises a buffer.
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