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Application and preparation of beta-sitosterol

A technology of sitosterol and pharmaceutical preparations, which is applied in the field of application of β-sitosterol and its preparations, and can solve problems such as unresearched

Active Publication Date: 2017-09-01
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research on the role of β-sitosterol in plant drought resistance.

Method used

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  • Application and preparation of beta-sitosterol
  • Application and preparation of beta-sitosterol
  • Application and preparation of beta-sitosterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The screening of embodiment 1β-sitosterol root application concentration

[0018] Test material: widely used 'Latinuo' white clover as the test material

[0019] Material cultivation: Soak the white clover seeds with 0.1% potassium permanganate solution for 10 minutes to disinfect, wash with deionized water for 3 times, press 1g / m 2 Seeds were sown in plastic pots with a length of 20 cm, a width of 15 cm, and a height of 5 cm filled with quartz sand, and placed in a light incubator for germination (temperature day / night was 23 / 19°C; duration was 12 hours; relative Humidity is 70%; light intensity is 700 μmol m -2 the s -1 ). After the seeds germinated for 7 days, the seedlings were continued to be cultivated with Hoagland nutrient solution until 30 days, and the materials with consistent growth were selected for subsequent experiments.

[0020] Experimental design: A total of 7 β-sitosterol concentration gradients were set up, which were: 0mg / L (control, direct drou...

Embodiment 2

[0025] Example 2 The screening of foliar spraying β-sitosterol concentration

[0026] Test material: Drought-sensitive 'Latinuo' white clover was used as the test material.

[0027] Material cultivation: Mix the pastoral soil and nutrient soil at a ratio of 1:1, add insecticides and fungicides and mix evenly, and place it outdoors for 3 days before use. Plant white clover in a pot with a diameter of 20cm and a depth of 25cm, and fill the pot with the same amount of mixed soil at 1g / m 2 Scatter the white clover seeds at a certain seeding rate, cover with shallow soil and water. The materials were placed in the greenhouse and cultivated for 3 months, and 250mL of Hoagland's complete nutrient solution was poured into each pot every week, and the routine management of fertilization, weeding, watering, and pests and diseases was uniformly carried out. The average temperature of the greenhouse is 23 °C, the relative humidity is 65%, the light time is 12 hours, and the light intens...

Embodiment 3

[0033] Example 3 Effect of foliar spraying of β-sitosterol on physiological indexes of drought resistance of white clover

[0034] Test material: Drought-sensitive 'Latinuo' white clover was used as the test material

[0035] Material cultivation: with embodiment 2

[0036] Design of the test: The optimal leaf spraying concentration 15mg / L screened out according to Experimental Example 2 is the spraying concentration of this experiment. There are 4 treatments in the test, and 4 independent repetitions are set for each treatment. They are respectively: 1. normal condition control (CK ): 18 days of normal culture in the greenhouse after spraying foliage without β-sitosterol as a control; ② CK+β-sitosterol: normal culture in the greenhouse after foliar spraying of 15 mg / L β-sitosterol 18 days; ③Direct drought stress: 18 days of natural drought in the greenhouse after spraying a solvent without β-sitosterol on the leaves; ④Drought + β-sitosterol: After spraying 15mg / L β-sitostero...

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Abstract

The invention relates to the technical field of agricultural production and particularly relates to application and a preparation of beta-sitosterol, in particular to application of beta-sitosterol to improvement in plant drought resistance. An experiment shows that the beta-sitosterol can effectively delay the wilting of white clover leaves under drought stress, relieve the growth inhibition caused by drought, reduce the degradation, the per-oxidation damage and the like of chlorophylls and significantly improve the drought resistance of white clovers. An application effect is not limited by the application time, and the drought-resistant effects on drought sensitive white clover varieties and drought-resistant white clover varieties are better. In addition, compared with ABA, SA, brassinolide and other exogenous additives, the beta-sitosterol is cheap in price, low in production cost and suitable for large-area popularization and application.

Description

technical field [0001] The invention relates to the technical field of agricultural production, in particular to the application of β-sitosterol and its preparation. Background technique [0002] With the frequent occurrence of extreme climates in the world, the uneven distribution of water resources and the reduction of available irrigation water resources, the impact of drought on crop yields around the world continues to expand, becoming one of the most important factors limiting plant growth and development. Since the middle of last century, the area affected by crop drought in my country has increased year by year. [0003] White clover (Trifolium repens L.) is a perennial high-quality forage grass belonging to the leguminosae genus Trifolium. One of the main leguminous pastures used is widely used in the establishment of artificial mixed grassland and the improvement of natural grassland. At the same time, due to the characteristics of strong creeping growth characte...

Claims

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Application Information

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IPC IPC(8): A01N45/00A01P21/00
CPCA01N45/00
Inventor 李州彭燕雍斌张艳彭丹丹程碧真张新全马啸黄琳凯闫艳红
Owner SICHUAN AGRI UNIV
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