Fermentation medium for Paenibacillus sp. 1-49 and optimization method thereof

A fermentation medium and Bacillus technology, which is applied to the fermentation medium of nitrogen-fixing Paenibacillus 1-49 and the field of optimization thereof, can solve the problems of unfavorable industrial production, low spore production, slow growth and the like, and achieves overcoming low spore yield. , improve the quality, enhance the effect of the application effect

Inactive Publication Date: 2017-09-05
DONGGUAN BAODE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Paenibacillus nitrogen-fixing grows slowly or even does not grow in ordinary medium, or the number of spores produced is very low, which is not conducive to industrial production

Method used

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  • Fermentation medium for Paenibacillus sp. 1-49 and optimization method thereof
  • Fermentation medium for Paenibacillus sp. 1-49 and optimization method thereof
  • Fermentation medium for Paenibacillus sp. 1-49 and optimization method thereof

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Effect test

Embodiment 1

[0029] The optimization method of the fermentation medium of the nitrogen-fixing Paenibacillus 1-49 of the present embodiment, it may further comprise the steps:

[0030] 1) Determination of nitrogen source

[0031] Under the condition of equal nitrogen content, peptone, yeast powder, bean cake powder, (NH 4 ) 2 SO 4 and different combinations as nitrogen sources to prepare liquid medium and solid medium, and other components remain unchanged, fermented and cultivated under the same conditions; other components of the liquid medium include raw materials of the following quality: 5g beef extract, 10g peptone, 5gNaCl, 1000mL Distilled water, pH 7.2-7.5; Other components of the solid medium include raw materials of the following quality: 5g beef extract, 10g peptone, 5g NaCl, 18g agar, 1000mL distilled water, pH 7.2-7.5.

[0032] Specifically, the fermentation culture is as follows: use an inoculation loop to inoculate 1 ring of Paenibacillus nitrogen-fixing 1-49 test tube str...

Embodiment 2

[0061] The optimization method of the fermentation medium of the nitrogen-fixing Paenibacillus 1-49 of the present embodiment, it may further comprise the steps:

[0062] 1) Determination of nitrogen source

[0063] Under the condition of equal nitrogen content, peptone, yeast powder, bean cake powder, (NH 4 ) 2 SO 4 and different combinations as nitrogen sources to prepare liquid medium and solid medium, and other components remain unchanged, fermented and cultivated under the same conditions; other components of the liquid medium include raw materials of the following quality: 5g beef extract, 10g peptone, 5gNaCl, 1000mL Distilled water, pH 7.2-7.5; Other components of the solid medium include raw materials of the following quality: 5g beef extract, 10g peptone, 5g NaCl, 18g agar, 1000mL distilled water, pH 7.2-7.5.

[0064] Fermentation culture is specifically as follows: use an inoculation loop to inoculate 1 ring of Paenibacillus nitrogen-fixing 1-49 test tube strains ...

Embodiment 3

[0094] The optimization method of the fermentation medium of the nitrogen-fixing Paenibacillus 1-49 of the present embodiment, it may further comprise the steps:

[0095] 1) Determination of nitrogen source

[0096] Under the condition of equal nitrogen content, peptone, yeast powder, bean cake powder, (NH 4 ) 2 SO 4 and different combinations as nitrogen sources to prepare liquid medium and solid medium, and other components remain unchanged, fermented and cultivated under the same conditions; other components of the liquid medium include raw materials of the following quality: 5g beef extract, 10g peptone, 5gNaCl, 1000mL Distilled water, pH 7.2-7.5; Other components of the solid medium include raw materials of the following quality: 5g beef extract, 10g peptone, 5g NaCl, 18g agar, 1000mL distilled water, pH 7.2-7.5.

[0097] The fermentation culture is specifically as follows: use an inoculation loop to inoculate 1 ring of Paenibacillus nitrogen-fixing 1-49 test tube stra...

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Abstract

The invention especially relates to a fermentation medium for Paenibacillus sp. 1-49 and an optimization method thereof, belonging to the technical fields of fermentation mediums and optimization of fermentation mediums. The fermentation medium is prepared from the following raw materials by mass: 15 to 20 g of bean cake powder, 5 to 8 g of maize starch, 6 to 10 g of cane sugar, 5 to 8 g of yeast powder, 0.2 to 0.5 g of MgSO<4>.7H<2>O, 1 to 3 g of CaCO<3>, 0.01 to 0.03 g of MgSO<4>.H<2>O, 0.25 to 0.75 g of KH<2>PO<4> and 1000 mL of distilled water with a pH value of 7.0 to 7.5. The optimization method comprises the following steps: determination of a nitrogen source; determination of a carbon source; determination of a carbon-nitrogen ratio; and determination of a pH value. The optimized fermentation medium for Paenibacillus sp. 1-49 in the invention overcomes the problem of low spore output of Paenibacillus sp., greatly increases spore output, enhances the application effect of Paenibacillus sp. 1-49 and effectively reduces production cost.

Description

technical field [0001] The present invention relates to the optimization technical field of fermentation medium and medium, relate in particular to a kind of fermentation medium of Paenibacillus nitrogen-fixing 1-49 and its optimization method. Background technique [0002] With the rapid rise of ecological agriculture in the world, due to the needs of food safety and environmental protection, the country has put forward a series of requirements and restrictions on fertilizers and pesticides. Therefore, the application of microbial fertilizers in agriculture has attracted more and more attention. Bacillus has many advantages such as storage resistance, strong stress resistance and salt-alkali tolerance. Therefore, Paenibacillus nitrogen-fixing plays a vital role in the preparation and application of microbial fertilizers. Paenibacillus sp. 1-49 is a highly efficient nitrogenase activity that can fix N2 in the air and convert it into NH4 for direct use by crops + , so as to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 陈三凤尹坤孙旭生刘剑敏杨国平王亚君杨盼盼李艳林敏沈世华张学贤官家发
Owner DONGGUAN BAODE BIOLOGICAL ENG
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