Analysis method for distinguishing real honey fromsyrup adulteratedhoney through chromatography mass spectrometry technology in combination with metabonomics method

Abstract

The invention discloses analysis method for distinguishing realhoney fromsyrup adulteratedhoney througha chromatography mass spectrometry technology in combination with a metabonomics method. The method comprises the following steps: performing pretreatment on a real honey sample and to-be-detectedsyrup adulteratedhoney with the same honey source of real honeyby adoptingan organic solventrespectively, separating and measuring chemical components in the samples subjected to pretreatment through an ultra-high performance liquid chromatography-high resolution mass spectrum, then preprocessing UHPLC-MS original data of the obtained real honey sample and the to-be-detected honey sample, and finally analyzing and distinguishing realhoney fromsyrup adulteratedhoney througha multivariate statistical analysis method PCA. The analysis method is superior to other detection methods, does not need qualitatively and quantificationally measuring one certain or certain targeted matter, and is a macroscopic and non-target method for varietydistinguishment.

Description

technical field [0001] The invention relates to a detection method for distinguishing real honey from syrup-adulterated honey by applying UHPLC-Q Exactive combined metabonomics technology, and belongs to the technical field of food adulteration identification. Background technique [0002] Honey is a natural sweet substance that bees collect nectar, secretions or honeydew from plants, mix with their own secretions, and fully brew. Honey has high nutritional value, but due to the high price and low output of honey, it makes Honey has become a main object of adulteration by illegal traders. In order to improve the production enthusiasm of beekeepers, protect the interests of consumers, support the fair competition of formal honey production enterprises, maintain the order of the honey market, and promote the healthy development of my country's honey industry, therefore It is of great significance and value to find the marker metabolites of real honey samples, so as to try to est...

AI Technical Summary

Problems solved by technology

The sensitivity of nuclear magnetic resonance (NMR) is low, and the metabolites we are concerned about in honey may be ignored, and NMR equipment is expensive and not suitable for daily monitoring

Near-infrared spectroscopy (NIRS) also has its fatal shortcomings: 1. It requires a large number of representative samples with known chemical values ​​to build models

Thus, the analysis of small batches of samples using near-infrared becomes impractical

2. The model needs to be updated continuously. Due to the change of the instrument state or the change of the standard sample, the model will also change accordingly.

3. The model is not universal, and the model of each instrument is different, which increases the limitation of use

4. The cost of modeling is high and the cost of testing is high

The various syrups currently adulterated with honey are very similar to honey in terms of physical and chemical properties, composition and content, and flavor. Therefore, the above-mentioned methods for measuring the quality of honey cannot detect honey adulterated with syrup

[0005] In summary, the existing technology lacks a fast, effective and intuitive method for identifying honey adulteration, and there is no combination of ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry combined metabolomics Studies on Determination of Marker Metabolites in Real Honey

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