Preparation method of jellyfish collagen emulsion
A jellyfish collagen and collagen technology, which is applied to skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as insignificant skin care effect, reduce water loss, facilitate absorption, and prevent arterial hardening effect
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[0021] A preparation method of jellyfish collagen emulsion, comprising the following steps:
[0022] Step 1: Clean the umbrella of fresh jellyfish with running water, cut it into pieces, put it into a beaker, add 10 times the volume of 0.1M sodium hydroxide solution, stir for 3 days, and wash until neutral; then add 10 times the volume of 0.5mol / L acetic acid solution, stirred for 2 days, washed to neutral;
[0023] Step 2: Dissolve the cleaned jellyfish in 8 times the volume of 0.5M acetic acid solution, add 1% by mass pepsin, stir and extract at 5°C for 3 days, centrifuge at 10,000rpm×1h, take out the supernatant, and put The supernatant is pepsin-promoted collagen crude solution. Add NaCl to the crude solution to a concentration of 0.9M, stir overnight, centrifuge at 10,000rpm×15min, take out the precipitate and dissolve it in 6 times the volume of 0.5M acetic acid, and transfer it to a dialysis bag for dialysis 2d, centrifuge at 10000rpm×15min, wash the obtained precipit...
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