Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for isolating and purifying total iridoid glycosides from ciliated papaya

A technology for total iridoid glycosides and ciliophora is applied in the field of separation and purification of total iridoid glycosides, which can solve the problem that the extraction process route is complicated and is not suitable for rapid separation and preparation of high-content iridoid glycosides. and other problems, to achieve the effect of excellent application prospect, easy operation and high yield

Active Publication Date: 2020-08-21
SICHUAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are only a few literatures on the extraction, separation and purification of iridoid glycosides in cilia, but due to the complexity of the extraction process, it is not suitable for rapid separation and preparation of high-content iridoid glycosides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A method for isolating and purifying total iridoid glycosides from cilia cilia

[0026] 1. Preparation method

[0027] (1) Take dry and pulverized ciliated mother-in-law powder (powder passed through a 40-mesh sieve), water-saturated n-butanol as the extraction solvent, the ratio of solid to liquid is 1:8, and extract at room temperature for 24 hours each time, three times in total, and combine the extraction solution to obtain a water-saturated n-butanol extract;

[0028] (2) wash with water, the volume ratio of water and step (1) gained extract is 1:1, and the number of times of washing is 3 times, and decompression reclaims n-butanol phase, obtains solid residue (being the crude iridoid glycosides of total iridoid glycosides) extract), dissolved in 80 times of water (per 1g of solid residue dissolved in 80ml of water), to obtain an aqueous solution;

[0029] (3) Carry out ethyl acetate extraction, the volume ratio of ethyl acetate to the aqueous solution...

Embodiment 2

[0032] Embodiment 2: A kind of method for separating and purifying total iridoid glycosides from cilia cilia

[0033] 1. Preparation method

[0034] (1) Take dry and pulverized ciliated mother-in-law powder (powder passed through a 40-mesh sieve), water-saturated n-butanol as the extraction solvent, the ratio of solid to liquid is 1:6, and extract at room temperature, each time for 48 hours, a total of three times, combined extraction solution to obtain a water-saturated n-butanol extract;

[0035] (2) wash with water, the volume ratio of water and step (1) gained extract is 1:5, and the number of times of washing is 5 times, and decompression reclaims n-butanol phase, obtains solid residue (being the crude iridoid glycosides of total iridoid glycosides) extract), dissolved in 80 times of water (per 1g of solid residue dissolved in 80ml of water), to obtain an aqueous solution;

[0036] (3) Carry out ethyl acetate extraction, the volume ratio of ethyl acetate to the aqueous ...

Embodiment 3

[0039] Embodiment 3: A kind of method for separating and purifying total iridoid glycosides from cilia cilia

[0040] 1. Preparation method

[0041] (1) Take dry and pulverized ciliated mother-in-law powder (powder passed through a 40-mesh sieve), water-saturated n-butanol as the extraction solvent, the ratio of solid to liquid is 1:10, and extract at room temperature for 12 hours each time, three times in total, and combine the extraction solution to obtain a water-saturated n-butanol extract;

[0042] (2) wash with water, the volume ratio of water and step (1) gained extract is 5:1, and the number of times of washing is 2 times, and decompression reclaims n-butanol phase, obtains solid residue (being the crude iridoid glycosides of total iridoid glycosides) extract), dissolved in 80 times of water (per 1g of solid residue dissolved in 80ml of water), to obtain an aqueous solution;

[0043] (3) Carry out ethyl acetate extraction, the volume ratio of ethyl acetate to the aqu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for isolating and purifying total iridoid glycosides from ciliated mother-in-law. The steps are as follows: (1) taking ciliated mother-in-law powder and extracting it with water-saturated n-butanol to obtain an extract; (2) washing with water , and recover the n-butanol phase under reduced pressure to obtain the crude extract of total iridoid glycosides, dissolve it in water to obtain an aqueous solution; (3) extract with ethyl acetate to obtain the extracted aqueous solution, and reclaim the aqueous phase under reduced pressure to obtain the total iridoid glycosides. The method of the invention can prepare high-purity total iridoid glycosides, and the method has high yield, simple operation, low energy consumption, high product purity and large output, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to a method for isolating and purifying active ingredients from cilia cilia, in particular to a method for isolating and purifying total iridoid glycosides. Background technique [0002] Veronica ciliata Fisch. (Veronica ciliata Fisch.) is an annual herb of the genus Scrophulariaceae. It is widely used in Tibetan medicine prescriptions and is currently distributed in China, Mongolia, and Central Asia. And Siberia and other regions, the whole herb can be used as medicine, which has the functions of clearing away heat and detoxification, expelling wind and dampness, and mainly treating hepatitis, cholecystitis, rheumatic pain, urticaria, etc. [0003] Studies have shown that ciliated mother-in-law contains a large amount of iridoid glycosides, which have a wide range of biological activities, such as antibacterial, anti-inflammatory, anti-tumor, liver protection and choleretic, anti-oxidation, cardiovascular system and nervous syste...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/80
CPCA61K36/80A61K2236/33A61K2236/333A61K2236/39
Inventor 唐琳华婉张士彦彭彤陈放
Owner SICHUAN UNIV