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Method for directly and quickly rooting tissue culture seedlings

A direct technology for tissue culture seedlings, applied in horticultural methods, botany equipment and methods, chemicals for biological control, etc., can solve problems such as laborious and laborious, waste of experimental materials, low transplanting survival rate, etc., and achieve reduction Low cost, easy operation, and high survival rate

Active Publication Date: 2017-09-19
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) It takes a lot of time and energy: the rooting of tissue culture seedlings on the medium first needs to prepare a large amount of rooting medium. In order to speed up the rooting speed, fresh medium needs to be replaced every two weeks. Remove the old medium remaining on the roots of the seedlings before they can be connected to fresh medium
When transplanting to the hole plate in the later stage, it is also necessary to remove all the remaining medium on the seedling roots, which is time-consuming and laborious.
[0005] (2) High experimental cost: the consumption of reagents used in the preparation of the medium is large, and the experimental cost is high
[0006] (3) Material waste, low rooting rate: in the process of changing the medium and transplanting the plug, it is inevitable that the root system of the plant will be damaged, resulting in a waste of experimental materials. The rooting rate of Chinese chestnut is 55.33%, and the rooting rate of cherry is 55.33%. Only 70-80%
[0007] (4) Due to the loss of training seedlings, the transplanting survival rate is low: when the rooted seedlings growing well on the rooting medium are transplanted to the hole trays, they need to experience environmental changes from the aseptic medium to the soil with bacteria matrix. It is a great test, it is easy to lose rooted seedlings and reduce the survival rate

Method used

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  • Method for directly and quickly rooting tissue culture seedlings
  • Method for directly and quickly rooting tissue culture seedlings
  • Method for directly and quickly rooting tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Make chestnut tissue culture seedling take root fast as follows:

[0040] 1. Take chestnut tissue-cultured seedlings grown in a culture bottle, grow well, have a plant height higher than 3 cm, a diameter larger than 2 mm, and have not taken root, and place them on filter paper; Cut obliquely with the knife, exposing the beveled stem.

[0041] 2. Take the jelly substance with a medicine spoon, and evenly apply it on the slope of the stem base and the stem, so that there is a jelly substance around the entire stem.

[0042] 3. Use a fruit pick to pierce a cylindrical hole with a diameter of about 3mm and a depth of 2 / 3 of the matrix block in the center of the matrix block that has absorbed enough water, and use a fruit pick to dip the jelly-like substance and spread it on the inner wall of the cylindrical hole as evenly as possible.

[0043] 4. Insert the tissue-cultured seedlings whose stems are coated with jelly-like substance directly into the matrix block coated with j...

Embodiment 2

[0047] With reference to the method described in Example 1, the tissue-cultured seedlings were rooted rapidly, the only difference being that the content of IBA in the jelly-like substance of step 3 and step 4 was 0.25% (w / w).

Embodiment 3

[0049] With reference to the method described in Example 1, the tissue-cultured seedlings took root rapidly, the only difference being that the content of IBA in the jelly-like substance of step 3 and step 4 was 0.28% (w / w).

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Abstract

The invention provides a method for directly and quickly rooting tissue culture seedlings. According to the rooting method, the basal stem of a tissue culture seedling is beveled, rooting hormone-containing gelatinoids is uniformly applied to the bevel of the basal stem, and the tissue culture seedling is inserted into a substrate to induce the tissue culture seedling to root in a culturing mode. The method has high rooting rate and high survival rate in late transplanting, is simple and convenient to operate, does not need cultivation on a rooting culture medium or preparation of massive rooting culture medium so as to greatly reduce cost, and does not need to clean the culture medium at the root part of the tissue culture seedling in the process of replacing a culture medium or subsequent transplanting so as to save time and labor. The variety and dosage of rooting hormone are optimized by the method, the optimized method is very suitable for ligneous plants, especially Chinese chestnuts and cherry, and the rooting rates of Chinese chestnuts and cherry are increased to 81-99 percent from 55 percent and 70 percent of a traditional rooting method.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for direct and rapid rooting of tissue culture seedlings. Background technique [0002] Plant tissue culture seedlings are complete plants cultivated under sterile and suitable artificial conditions using explants based on the totipotency of plant cells. Rooting is to let the tissue culture seedlings grow roots. At present, the common rooting method of plant tissue culture seedlings at home and abroad is the rooting medium rooting method, that is, on the basis of 1 / 2MS and WPM basal medium, add plant hormones IBA or NAA with different concentrations, and then the tissue culture seedlings Place it on the rooting medium for culture, and induce the tissue culture shoots to take root. The medium rooting method has been generally applied on the rooting of plant tissue culture seedlings, and in addition, no other innovative rooting methods have been seen. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N43/38A01P21/00
CPCA01H4/008A01N43/38
Inventor 曹庆芹秦岭邢宇李晓韩远芳
Owner BEIJING UNIV OF AGRI