Anaerobic culture bottle inflatable vacuum cleaning system

Abstract

The invention discloses an anaerobic culture bottle inflation and vacuumizing cleaning system. The system comprises an anaerobic culture medium rapid split charging module, a gas component matching module, a branch terminal culture bottle cleaning module and a vacuumizing module. The gas component matching module is connected with the anaerobic culture medium rapid split charging module and the branch terminal culture bottle cleaning module. The vacuumizing module is connected with the branch terminal culture bottle cleaning module. The anaerobic culture medium rapid split charging module and the branch terminal culture bottle cleaning module are connected with a culture bottle. The anaerobic culture bottle inflation and vacuumizing cleaning system has the advantages that the system has the adjustable anaerobic degree and the pressure index, and can achieve free matching of N2 / CO2 and H2 quantitative supply.

Description

technical field [0001] The present invention mainly relates to the technical field of anaerobic operation device manufacturing, in particular to a controllable N 2 / CO 2 Proportion and quantitative supply of H 2 The anaerobic culture bottle is inflated and vacuumed to clean the system. Background technique [0002] There are natural anaerobic / microaerobic environments in nature, and microorganisms living in these environments are sensitive to the oxygen concentration in the culture environment. Survival and laboratory culture are critical. In addition, carbon dioxide and hydrogen are effective carbon sources and electron donors, respectively, for the growth and metabolism of microorganisms (especially anaerobic microorganisms), and their quantitative supply is crucial for the successful cultivation of microorganisms in laboratory conditions. [0003] At present, the commonly used anaerobic operating system such as anaerobic operation box and simply blowing nitrogen into ...

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Problems solved by technology

[0003] At present, the commonly used anaerobic operating system such as anaerobic operation box and simply blowing nitrogen into the culture medium have limitations in the following aspects: 1. It is difficult to ensure a strict anaerobic environment; 2. It is impossible to achieve N in the box. 2 / CO 2 The free ratio and quantitative supply of H 2 ; 3. The anaerobic degree and pressure index in the anaerobic culture bottle cannot be regulated

Therefore, it cannot meet the requirements of different experimental designs and the needs of different types of microbial growth for anaerobic environment

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