Two-step degradation method for pectin in tobacco sheets

A pectin and tobacco technology, applied in the directions of tobacco, tobacco treatment, microorganism-based methods, etc., can solve the problems of insufficient degradation, difficult control of the degradation process, and poor targeting of pectin degradation.

Inactive Publication Date: 2017-09-19
CHINA TOBACCO HENAN IND +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity and diversity of the pectin structure in the tobacco sheet, pectinases in the prior art generally have the defects that the degradation of pectin in the tobacco sheet is not targeted, the degradation is not sufficient, and the degradation process is difficult to control. Urgent need to explore new pectinases and their application in tobacco sheet processing

Method used

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  • Two-step degradation method for pectin in tobacco sheets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The pectinase prepared by fermentation of Aspergillus honey provided in this example is obtained through the following production steps.

[0058] (1) Activation of the strains, pick the fermentation strains from the preservation medium, inoculate them into the activation medium and activate them at 30°C for about 3 days;

[0059] The fermentation strain is specifically the Aspergillus honeysuckle with the preservation number 40926 preserved by the China Industrial Microorganism Collection Center (CICC); the strains used in this example were purchased from the corresponding preservation unit;

[0060] The activation medium is a PDA medium; when inoculating the activation medium after picking bacterial strains from the preservation medium, the streaking method is used to inoculate;

[0061] The PDA culture medium is prepared according to conventional methods, specifically: 1.0L of potato extract, 20.0g of glucose, 15.0g of agar, and natural pH;

[0062] The preparation m...

Embodiment 2

[0074] The crude enzyme liquid prepared in Example 1 was applied to the two positions of the secondary leachate and the mixed pulp on the production line of China Tobacco Tobacco in Henan Province respectively (the process position is as follows: figure 1 shown), used to degrade the pectin component in the leach solution and slurry.

[0075] The specific method of use is to use the crude enzyme liquid of pectinase prepared in Example 1 (the enzyme activity is 2432.63U / mL, theoretically, the crude enzyme liquid with any enzyme activity prepared in Example 1 can be used to degrade pectin application, but in order to determine better degradation conditions and the actual improvement effect on tobacco, so only the crude enzyme solution prepared in Example 1 with an enzyme activity of 2432.63U / mL was used as an example for the experiment) to carry out appropriate dilution, and then the crude enzyme solution after dilution Enzyme solution is based on different material ratios (the r...

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Abstract

The invention belongs to the technical field of tobacco preparation, and particularly relates to a two-step degradation method for pectin in tobacco sheets. According to the method, specific strains are firstly fermented to obtain crude enzyme with highest pectic enzyme activity, the crude enzyme is used for a tobacco sheet process production line by a 'two-step method', and leaching solution of the production line and the content of the pectin in slurry are reduced. Preliminary tests show that the enzyme activity of the obtained crude enzyme can reach 2432.63U / mL, and the content of the pectin in the tobacco sheets can be reduced to 1.52% from 2.00% after the crude enzyme is further used for the tobacco sheet production line. After the treated tobacco sheets are used for cigarettes, sensory smoking evaluation indicates that compared with untreated samples, cigarette samples added with the tobacco sheets after enzymolysis are more delicate in fragrance, sufficient in strength and better in combustibility, sweet feeling is enhanced, irritation is reduced, fragrance amount is increased, offensive odor is decreased, and accordingly, the smoking quality of tobaccos is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of tobacco preparation, and in particular relates to a two-step degradation method for pectin in tobacco sheets. Background technique [0002] Paper-Process Reconstituted Tobacco is a product of recycling tobacco resources. It uses some tobacco waste and leftovers, such as tobacco stems, tobacco leaf fragments, tobacco powder, etc., as raw materials, and undergoes extraction, concentration, separation, beating, refining, copying, drying, and flavoring processes to make tobacco leaf thin slices. , used as cigarette filler. Paper-making tobacco sheet not only has advantages in improving the structural strength and combustion performance of tobacco leaf products and reducing the amount of nicotine and tar in cigarette smoke, but also can reduce the unit consumption of tobacco leaves and save production costs. One of the important results. In recent years, the combined application of biotechnology and paper-m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/18C12N9/24C12N9/88A24B15/20C12R1/66
CPCA24B15/20C12N9/18C12N9/2402C12N9/88C12Y301/01011C12Y302/01015C12Y402/02002
Inventor 许春平郝辉陈顺辉邱建华李国政宋金勇张展周浩余金伟姜宇王充冉盼盼
Owner CHINA TOBACCO HENAN IND
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