Bladder cancer screening and detecting kit

Abstract

The invention mainly belongs to the field of onco molecular biology and specifically relates to a bladder cancer screening and detecting kit. The kit is applied to detecting oligonucleotide of cDNA of a related gene of bladder cancer. The kit contains a specific prime pair, the specific primer pair contains an upstream primer and a downstream primer, a sequence of the upstream primer is shown as SEQ ID No.1, and a sequence of the downstream primer is shown as SEQ ID No.2. The kit can detect the oligonucleotide of the cDNA of the related gene of the bladder cancer, and KMT1A can serve as a novel marker and be applied to auxiliary diagnosis of the bladder cancer.

Description

technical field [0001] The invention mainly belongs to the field of tumor molecular biology, and in particular relates to a bladder cancer screening and detection kit. Background technique [0002] Bladder cancer is the most common malignant tumor in the urinary system tumors. In my country, the incidence rate of bladder cancer is 6.61 / 100,000, ranking first among urinary system tumors. Risk factors for bladder cancer include smoking, aromatic amine carcinogens, arsenic, chronic bladder infection, ionizing radiation, and phenacetin abuse. According to the pathological features, bladder cancer is clinically divided into superficial type (70%) and invasive type (30%). Poor prognosis and easy recurrence are the biggest characteristics of bladder cancer, and effective treatment largely depends on the early detection and treatment of bladder cancer. [0003] Currently, the diagnosis of bladder cancer mainly relies on cystoscopy and urine cytology. The former is the gold stand...

AI Technical Summary

Problems solved by technology

Elevated levels of urinary BTA have certain early diagnostic value for bladder cancer. Miyake et al. collected urine samples from 126 patients with hematuria (64 cases of bladder cancer group and 62 cases of control group) and used the BTA detection kit (BTA and ) detected the BTA level in the urine sample, and the results showed that the positive rate of the bladder cancer group was 72%, but 47% of the patients in the control group were also positive results. The sensitivity and specificity of BTA to bladder cancer were respectively 72% and 53%, so BTA detection is helpful for the early diagnosis of bladder cancer, but it is susceptible to hematuria and false positive results

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