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A method for tissue culture and rapid propagation of Alba vine

A technique for tissue culture and rapid propagation of Alba vine, which is applied in the field of plant tissue culture, and can solve problems such as the tissue culture technology for the tissue culture of Albino edulis and the patent application for tissue culture and rapid propagation of the elixir. , to achieve the effects of favorable development and utilization, short cycle and low cost

Active Publication Date: 2020-07-24
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the tissue culture technology of Alba vine stems at home and abroad, and there has been no application for a patent for tissue culture and rapid propagation of Alba vine stems

Method used

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  • A method for tissue culture and rapid propagation of Alba vine

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Embodiment 1

[0024] A method for tissue culture and rapid propagation of the yao medicine wing stem Alba vine, comprising the following steps:

[0025] (1) Collection of explants: The explants were selected in the wild from the sun-nosed stems of the genus Alpinia pterygium with no obvious diseases. After collection, the explants were treated with water retention and moisturizing immediately and brought back to the laboratory in time.

[0026] (2) Induction of explants: when the explants were collected in step (1) and returned to the laboratory, they were rinsed under tap water overnight. After soaking in 75% alcohol solution for 15 seconds in the ultra-clean workbench, immediately rinse it with sterile water for 3 times. After soaking in 0.1% mercuric chloride solution for 10 minutes, immediately rinse with sterile water 5 times, dry the surface moisture of explants with sterile filter paper, cut into 1.5cm segmented stems and inoculate them into adventitious bud induction culture cultur...

Embodiment 2

[0032] A method for tissue culture and rapid propagation of the yao medicine wing stem Alba vine, comprising the following steps:

[0033] (1) Collection of explants: The explants were selected in the wild from the sun-nosed stems of the genus Alpinia pterygium with no obvious diseases. After collection, the explants were treated with water retention and moisturizing immediately and brought back to the laboratory in time.

[0034] (2) Induction of explants: when the explants were collected in step (1) and returned to the laboratory, they were rinsed under tap water overnight. After immersing in 75% alcohol solution for 20 seconds in the ultra-clean workbench, immediately rinse it with sterile water 5 times. Immediately after soaking in 0.1% mercuric chloride solution for 15 minutes, rinse with sterile water 7 times, dry the surface moisture of the explants with sterile filter paper, cut into 2.0 cm segmented stem segments and inoculate them into adventitious bud induction cult...

Embodiment 3

[0040] A method for tissue culture and rapid propagation of the yao medicine wing stem Alba vine, comprising the following steps:

[0041](1) Collection of explants: The explants were selected in the wild from the sun-nosed stems of the genus Alpinia pterygium with no obvious diseases. After collection, the explants were treated with water retention and moisturizing immediately and brought back to the laboratory in time.

[0042] (2) Induction of explants: when the explants were collected in step (1) and returned to the laboratory, they were rinsed under tap water overnight. Immediately rinse 4 times with sterile water after immersing in 75% alcohol solution for 18 seconds in the ultra-clean workbench. After soaking in 0.1% mercuric chloride solution for 12 minutes, immediately rinse with sterile water 6 times, dry the surface moisture of the explants with sterile filter paper, cut into 1.6 cm segmented stem segments and inoculate them into adventitious bud induction culture ...

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Abstract

The invention relates to the technical field of plant tissue culture, in particular to a tissue culturing and rapid propagation method for the yao medicine vitaceae cissus hexangularis. The tissue culture and rapid propagation method for yao medicine vitaceae cissus hexangularis includes the following steps that 1, explants are collected; 2, the explants are induced; 3, subculture is conducted; 4, roots are cultured; 5, transplanting is conducted. The tissue culturing and rapid propagation method for the yao medicine vitaceae cissus hexangularis has the advantages of low costs, short circles and the simple operation, can be directly used for industrialized production for saplings of vitaceae cissus hexangularis, and is beneficial for the development and utilization of the yao medicine vitaceae cissus hexangularis.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Rhizoma japonica. Background technique [0002] Cissux hexangularis Thorel ex Planch (Cissux hexangularis Thorel ex Planch) is the dry stem of the genus Cissux hexangularis Thorel ex Planch. , stilbenes, alkaloids, coumarins, and steroidal compounds have the effects of expelling wind and dampness, promoting blood circulation and dredging collaterals, and are commonly used in Yao people for bruises, rheumatic joint pain, fractures, sores, swelling, and dysentery , Snake bites and other diseases. [0003] There are more than 200 species of vines in the world, and there are 15 species in my country, mainly including vines with hairy leaves, vines with wing stems, and vines with halberd leaves, which are mainly distributed in southern provinces. Alpinia genus plants have shown unique advantages in cardiov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 史艳财唐健民邹蓉蒋运生熊忠臣韦记青
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI