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Preparation method of nano pearl powder/c-ha composite scaffold

A technology of nano pearl powder and composite scaffold, which is applied in the field of bioengineering, can solve the problems that hydroxyapatite is not easy to degrade, and the degradation speed of tricalcium phosphate cannot be regulated, and achieves the advantages of proliferation, good biological activity and good wettability. Effect

Active Publication Date: 2020-08-04
海口市人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, hydroxyapatite is not easy to degrade in the body, and the degradation rate of tricalcium phosphate cannot be controlled, while natural biomaterials have good biocompatibility and biodegradability, and can promote the growth of biological signals by inducing the release of biological signal factors. Osteocyte adhesion, proliferation and differentiation

Method used

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  • Preparation method of nano pearl powder/c-ha composite scaffold
  • Preparation method of nano pearl powder/c-ha composite scaffold
  • Preparation method of nano pearl powder/c-ha composite scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0053] 1. Reagents, as shown in Table 2

[0054] Table 2

[0055] Reagent company and model micron pearl powder Hainan Nanjing Run Pearl Biotechnology Co., Ltd. Sodium hyaluronate H107141, Aladdin Chitosan C105803, Aladdin acetic acid Analytical pure, Aladdin

[0056] 2. The instrument is shown in Table 3

[0057] table 3

[0058]

[0059]

[0060] 3. Operation steps

[0061] 1) Grind the micron pearl powder into nano pearl powder by mechanical ball milling with a nano ceramic sand mill disperser, dry and sterilize;

[0062] 2) Dissolve 4wt% chitosan and 1wt% hyaluronic acid into 1% acetic acid solution respectively, stir for 5min at a speed of 2000rpm in a Thinky mixer, and place it at room temperature for 24h to obtain chitosan acetic acid solution and hyaluronic acid acid acetic acid solution;

[0063] 3) After 24 hours, mix the chitosan acetic acid solution and the hyaluronic acid acetic acid solution, and stir for 5 m...

Embodiment 1 Embodiment 5

[0070] 2. Experimental reagents and instruments

[0071] The same as the reagent of embodiment and instrument

[0072] 1) Experiment 1: Determination of the surface morphology of the composite scaffold

[0073] 1. Observation of the surface morphology of composite scaffolds by scanning electron microscopy

[0074] Cut the sample into thin slices with a width of 3 mm to make the surface of the sample as flat as possible. After drying with a vacuum freeze dryer, stick the samples on the conductive adhesive in turn, mark the upper left corner of the conductive adhesive, and spray gold on the surface of the sample for 60 seconds. Then open the sample chamber of the scanning electron microscope (MIRA3, Czech Republic), fix the sample to be observed, vacuumize, and directly observe the cross-sectional morphology with the scanning electron microscope.

[0075] 2. Conclusion

[0076] Such as figure 1 As shown, the scaffold is highly porous with uniformly distributed pores and goo...

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Abstract

The invention discloses a preparation method of nano-pearl powder / C-HA composite scaffold. The preparation method comprises the following steps: firstly, dissolving chitosan and hyaluronic acid into an acetic acid solution with concentration of 1% respectively, stirring at 2000rpm for 5min, placing at room temperature for 24 hours, and obtaining a chitosan acetic acid solution and a hyaluronic acid acetic acid solution; secondly, 24 hours later, mixing the chitosan acetic acid solution and the hyaluronic acid acetic acid solution, and stirring at 2000rpm for 5 min; thirdly, adding the nano-pearl powder with the weight percentage of 1 to 25%, stirring at 2000 rpm for 5min; fourthly, putting into a -20 DEG C refrigerator for 24h, then freeze drying for 24h, and obtaining the composite scaffold. The technical problem to be solved by the invention is to provide a preparation method of nano-pearl powder / C-HA composite scaffold which has a suitable pore structure, is advantageous for cell proliferation, blood vessel growth and transport of nutrients, has good biological activity, biocompatibility and biodegradability, and can adapt to the specific shape of bone defects.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a preparation method of nano pearl powder / C-HA composite scaffold. Background technique [0002] Bone defects have become a global health problem. With the aging of the population and the development of society, the repair of bone defects has become an important clinical topic and social demand. At present, the clinical treatment of bone defects mainly relies on allogeneic bone grafting or autologous bone grafting, but there are many limitations in these two treatment methods. For a long time, autologous bone transplantation has been recognized as the gold standard of bone transplantation, but there are disadvantages such as limited amount of available bone, complications at the donor site, and easy absorption of transplanted bone blocks; allogeneic bone transplantation has immune rejection and transmission of infectious diseases risks of. [0003] With the development of medicin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44A61L27/50A61L27/54A61L27/56A61L27/58
CPCA61L27/44A61L27/50A61L27/54A61L27/56A61L27/58A61L2300/30A61L2300/412A61L2430/02C08L5/08
Inventor 徐普李晓妮
Owner 海口市人民医院
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