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Detection method of 1-deoxynojirimycin (DNJ) in mulberry leaf extract

A technology of deoxynojirimycin and mulberry leaf extract, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex operation process, poor applicability, and difficult conditions, so as to achieve good popularization, sample addition and recovery High efficiency, simple and convenient operation

Inactive Publication Date: 2017-09-22
JIANGSU NAIQUE BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Chinese patent CN201310015381.6 and Chinese patent CN201410108293.5 disclose the above two methods, which have two disadvantages: on the one hand, the pre-column derivatization of DNJ is complicated and the conditions are not easy to grasp; on the other hand, the price of the fluorescence detector Expensive and rarely configured in general laboratories; therefore, this method is not easy to popularize
[0006] In addition, Chinese patent CN201110309072.0 discloses a method for directly measuring the content of DNJ using a reversed-phase high-efficiency liquid phase-ultraviolet detector using the method of terminal absorption; More samples are less suitable

Method used

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  • Detection method of 1-deoxynojirimycin (DNJ) in mulberry leaf extract
  • Detection method of 1-deoxynojirimycin (DNJ) in mulberry leaf extract

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Effect test

Embodiment 1

[0036] A method for detecting the content of 1-deoxynojirimycin in a mulberry leaf extract, specifically using a reversed-phase high performance liquid chromatography-evaporative light scattering detection method, specifically comprising the following steps:

[0037] (1) Liquid chromatography conditions and system adaptability test: amino-bonded silica gel was used as filler, Shimadzu InertSustainNH 2 Chromatographic column (4.6mm×250mm, 5μm); acetonitrile solution is used as mobile phase A, 6.5mmol ammonium acetate solution is used as mobile phase B; room temperature is 20°C, relative humidity is 35%, column temperature is 30°C, flow rate is 1mL / min, The injection volume is 20 μL; the Chromachem evaporative light scattering device is used for detection, the attenuation is set to 5, the sensitivity is medium, the nebulization temperature is 30°C, and the evaporation temperature is 45°C; the gradient elution is carried out according to the provisions in Table 1:

[0038] Table ...

Embodiment 2

[0044] A method for detecting the content of 1-deoxynojirimycin in a mulberry leaf extract, specifically using a reversed-phase high performance liquid chromatography-evaporative light scattering detection method, specifically comprising the following steps:

[0045] (1) Liquid chromatography conditions and system adaptability test: amino-bonded silica gel was used as filler, Shimadzu InertSustainNH 2 Chromatographic column (4.6mm×250mm, 5μm); acetonitrile solution as mobile phase A, 6.5mmol ammonium acetate solution as mobile phase B; room temperature 20°C, relative humidity 35%, column temperature 25°C, flow rate 0.8mL / min , the injection volume was 20 μL; detection was performed with a Chromachem evaporative light scatterer, the attenuation was set to 5, the sensitivity was medium, the nebulization temperature was 35°C, and the evaporation temperature was 55°C; gradient elution was carried out according to the provisions in Table 2:

[0046] Table 2 Mobile phase elution gra...

Embodiment 3

[0052] A method for detecting the content of 1-deoxynojirimycin in a mulberry leaf extract, specifically using a reversed-phase high performance liquid chromatography-evaporative light scattering detection method, specifically comprising the following steps:

[0053] (1) Liquid chromatography conditions and system adaptability test: amino-bonded silica gel was used as filler, Shimadzu InertSustainNH 2 Chromatographic column (4.6mm×250mm, 5μm); acetonitrile solution as mobile phase A, 6.5mmol ammonium acetate solution as mobile phase B; room temperature 20°C, relative humidity 35%, column temperature 40°C, flow rate 1.2mL / min , the injection volume was 20 μL; the Chromachem evaporative light scatterer was used for detection, the attenuation was set to 5, the sensitivity was medium, the nebulization temperature was 40°C, and the evaporation temperature was 55°C; gradient elution was carried out according to the provisions in Table 3:

[0054] Table 3 Mobile phase elution gradien...

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Abstract

The invention discloses a detection method of 1-deoxynojirimycin (DNJ) in a mulberry leaf extract. The detection method comprises the following steps: adopting a reversed-phase high-performance liquid chromatography-evaporative light scattering detection method, selecting an amino column to separate the 1-deoxynojirimycin in the mulberry leaf extract, adopting an evaporative light-scattering detector for matched detection and calculating the content of the 1-deoxynojirimycin by adopting an external standard two-point method. The detection method disclosed by the invention is simple and convenient to operate and good in precision degree, reproducibility and stability; pre-column derivatization and marking are not required to be performed on the 1-deoxynojirimycin before use; a detection instrument is moderate in price, good in popularization and high in sample recovery rate; the detection method is used for the detection on the content of the 1-deoxynojirimycin in the mulberry leaf extract, so that the effective method is provided for quality control on the mulberry leaf extract.

Description

technical field [0001] The invention relates to the technical field of extract detection, in particular to a method for detecting the content of 1-deoxynojirimycin in mulberry leaf extract. Background technique [0002] Mulberry leaves are the dry leaves of the Moraceae plant mulberry, cultivated or wild, and have strong adaptability to soil, and are mostly planted in most parts of the country. Mulberry leaves are bitter in the mouth, sweet, and cold in nature. Studies have found that mulberry leaves have anti-inflammatory and hypoglycemic effects pharmacologically, and can lower blood fat and diuresis. [0003] 1-Deoxynojirimycin (DNJ) is a strong α-glucosidase inhibitor, which inhibits α-glucosidase in the small intestine to decompose disaccharides such as sucrose and maltose that the human body decomposes from starchy foods into Glucose can significantly inhibit the sharp rise of blood sugar after eating; the synergistic effect of 1-deoxynojirimycin (DNJ) and mulberry l...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 易铭钟慧李晔金显友魏元刚杨永安
Owner JIANGSU NAIQUE BIOLOGICAL ENG
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