Multispectral microscopic imaging system and microscope

Abstract

The present invention discloses a multispectral microscopic imaging system and a microscope. The multispectral microscopic imaging system comprises an amplifying device, a spatial mask, a double-lens 4f system, an optical grating and an acquisition device; the amplifying device is used for amplifying light which is emitted by a sample sheet to be measured due to excitation; a distance between the amplifying device and the sample sheet to be measured is not greater than a predetermined distance; the spatial mask is arranged at one side of the amplifying device; a distance between the spatial mask and the amplifying device is properly set so that all the information of light that passes through the amplifying device can be irradiated on the spatial mask; the double-lens 4f system is arranged at one side of the spatial mask; a distance between the double-lens 4f system and the spatial mask is the distance of one focal length; the optical grating is arranged on the Fourier surface of the double-lens 4f system; and the acquisition device is arrange at one side of the double-lens 4f system and is used for acquiring spectral information. With the multispectral microscopic imaging system and the microscope of the present invention adopted, the rate of multi-spectral microscopic imaging can be obviously improved, light efficiency can be also effectively improved, and the quality of shot pictures can be obviously improved.

Description

technical field [0001] The invention relates to the technical field of computational microscopy, in particular to a multispectral microscopic imaging system and a microscope. Background technique [0002] Fluorescence microscopy has a wide range of applications in the life sciences. For example, when studying the transfer characteristics of a certain living structure, it is usually necessary to implant a certain fluorescent protein into it and track it. At the same time, changes in the autofluorescence of life structures can also reflect changes in the nature of life to some extent. Therefore, research on fluorescence microscopy will be able to greatly promote the development of life sciences. [0003] Conventional fluorescence microscopy techniques mainly include confocal fluorescence microscopy, which utilizes specific excitation light to excite fluorescence on the fluorescent sample set on the focal level, and uses a camera sensor at the acquisition end to collect the f...

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Problems solved by technology

[0004] Among the related technologies, the main disadvantage of multispectral microscopy is that while improving the spectral resolution, the field of view, spatial resolution or temporal resolution is often sacrificed

For example, the method of using a narrow-band filter can obtain the spectral information of the specified band every time in a single acquisition, but it needs to replace the hardware facilities every time, increase the acquisition time, and it is difficult to obtain a higher spectral resolution

Higher spectral resolution can be obtained by using grating light splitting and grouping camera pixels, but this technique reduces spatial resolution by the same factor as spectral resolution is obtained

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