Simple chip device easy to disassemble and assemble and suitable for joint detection of typical tumor markers

Abstract

The invention relates to a simple chip device easy to disassemble and assemble and suitable for joint detection of typical tumor markers, and belongs to the field of analytical test. PDMS, namely polydimethylsiloxane, has both advantages and serial problems to be solved when used to make a substrate of a microfluidic chip for multichannel joint detection of typical tumor markers; the simple chip device herein is intended to solve the serial problems. The simple chip device is characterized in that PDMS with original ecological surface is selected for a substrate, a hinged clamp with an attached miniature ultrasonic transducer is manually fastened and positioned to a positon close to a sample flow terminal of the microfluidic chip, and an ultrasonic reducer is fitted to a sample injecting end of the microfluidic chip; therefore, ultrasonic strength is gradually decreased fast within a short range, an interfacial tension difference occurs between the two ends of the chip, and a sample flow is driven to flow along an originally hydrophobic capillary passage to the terminal. The simple chip device has no need for a miniature pump.

Description

technical field [0001] The invention relates to a simple disassembled and assembled chip device for combined detection of typical tumor markers, which belongs to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive ...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical problem i

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