Automatic focusing method of CCD (charge coupled device) biological chip fluorescent scanner
A fluorescence scanner, biochip technology, applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve problems such as difficulty in instrument focusing, achieve a high degree of automation, keep the size of the field of view unchanged, and fast.
Active Publication Date: 2017-09-29
FUZHOU UNIV
View PDF13 Cites 6 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] The object of the present invention is to provide a kind of automatic focusing method of CCD biological chip fluorescence scanner, this method adopts the total signal-to-noise ratio SNR of the fluorescent pixel ratio p of fluorescent point and fluorescent dot matrix T , which are used as the evaluation function of coarse focusing image sharpness and fine focusing image sharpness evaluation function respectively, which solves the problem of difficult focusing of the instrument; compared with the manual focusing process, the automatic focusing design has high accuracy and fast speed and the advantages of a high degree of automation
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0024] The technical solution of the present invention will be specifically described below in conjunction with the accompanying drawings.
[0025] An automatic focusing method of a CCD biochip fluorescent scanner of the present invention provides an automatic focusing device of a CCD biochip fluorescent scanner, the automatic focusing device of the CCD biochip fluorescent scanner comprises a light source, a CCD camera, Imaging lens, motor, single-chip microcomputer, host computer, described method: first, utilize light source to carry out fluorescence excitation to biological chip, adjust the focal length of imaging lens to initial position through single-chip microcomputer control motor, then utilize host computer to CCD camera collects through imaging lens The fluorescence image of the biochip is evaluated for rough focus image definition and fine focus image definition evaluation, and realizes coarse adjustment and fine adjustment of the focal length of the imaging lens, th...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to an automatic focusing method of a CCD (charge coupled device) biological chip fluorescent scanner, and provides an automatic focusing device of the CCD biological chip fluorescent scanner. The method comprises the following steps of firstly, using a light source to perform fluorescent excitation on a biological chip, and enabling a single chip microcomputer to control a motor to adjust the focal length of an imaging lens to the initial position; then, utilizing an upper computer to perform rough focusing image definition evaluation and fine focusing image definition evaluation on a fluorescent image of the biological chip collected by the imaging lens of a CCD camera, and completing the rough focusing and fine focusing on the focal length of the imaging lens, so as to complete the adjusting of the focal length of the imaging lens. The method has the advantages that the fluorescent pixel ratio p of a fluorescent dot and a total signal and noise ratio SNRT of a fluorescent dot matrix are respectively used as a rough focusing image definition evaluation function and a fine focusing image definition evaluation function, so that the problem of difficulty in focusing of instruments is solved; compared with the manual focusing process, by adopting the automatic focusing design, the accuracy is high, the speed is high, and the automation degree is high.
Description
technical field [0001] The invention relates to an automatic focusing method of a CCD biochip fluorescence scanner. Background technique [0002] The patent (application number: 201220260024.7) proposes an automatic focusing system for linear array CCD, which is characterized in that automatic focusing is realized by moving the camera lens (changing the object distance) and collecting images with CCD. The patent (application number: CN201610597471.4) proposes a high-power LED microarray chip fluorescence detection method, which is characterized in that the biochip is excited by a designed ring-shaped LED lighting array, and then the emitted fluorescence is collected by a CCD camera. The patent (application number: CN02252864.4) proposes a design of a CCD biochip detector with a uniform illumination system, which filters the white light emitted by the xenon lamp into parallel monochromatic light through optical elements, and then obliquely incidents it on the biochip Fluores...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/645G01N21/6486
Inventor 陈建国高跃明姜海燕甘振华杜民王帆
Owner FUZHOU UNIV