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Soybean small peptide, preparation method and applications thereof

A technology of soybean small peptides and microbial strains, applied in the biological field, can solve the problems of osmotic pressure imbalance in the body, excessive intake of nutrients, etc., achieve low fishy smell, strong protein decomposition ability, and improve the effect of comprehensive utilization value

Inactive Publication Date: 2017-10-03
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, modern people’s lifestyle emphasizes fast, efficient and practical. For convenience and sensory pleasure, a large amount of polished rice noodles and other convenient foods such as high-sugar carbonated sodas are developed and consumed. These single-nutrition diets are full of salt , sugar, energy and acid, long-term consumption and adequate drinking water will lead to excessive intake of nutrients and imbalance of osmotic pressure in the body

Method used

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  • Soybean small peptide, preparation method and applications thereof
  • Soybean small peptide, preparation method and applications thereof
  • Soybean small peptide, preparation method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1. Isolation, characterization, cultivation and preservation of Bacillus cereus of the present invention

[0044] 1.1 Strain isolation

[0045] Weigh 10g of tempeh powder into a 250mL Erlenmeyer flask, add 100mL of sterile normal saline, shake for 2-4 hours, settle still to obtain the supernatant, take out 10mL for dilution, and dilute the bacterial solution to 10 -7 , take 10 respectively -1 、10 -3 、10 -3 、10 -7 These several gradients were subjected to plate coating experiments, spread on casein medium, and cultured at 37°C for 24 hours to observe whether a transparent circle was produced. Pick 9 single colonies that produce transparent circles, streak them in the separation medium, purify the strains, and finally preserve the 9 strains on the slant, and then carry out the enzyme activity test. According to the size of the transparent circle and the size of the enzyme activity, the bacterial strain with the highest enzyme activity is selected, that is,...

Embodiment 2

[0069] Embodiment 2. Preparation of soybean small peptide

Embodiment 21

[0071] Soy small peptides are prepared according to the following steps:

[0072] (a) Weighing the twice-soaked soybean meal, adding water, autoclaving, inoculating the Bacillus cereus of the present invention and fermenting in a fermenter for 72 hours in an airtight manner;

[0073] (b) add water to the fermented soybean meal obtained in step (a), add thermolysin (thermolysin) to carry out enzymolysis after being fully infiltrated, and then ultrasonically extract to obtain an enzymolysis solution, and the amount of thermolysin is 5 ‰ mass parts (g / kg);

[0074] (c) Pass the obtained enzymatic solution in step (b) through a 400-mesh filter cloth to remove particulate matter, add activated carbon and Florisil and let it stand for half an hour to purify, then filter through a plate and frame filter press to remove activated carbon and Florisil Silica, to obtain debitter deodorization and partly decolorized soybean protein serum, the consumption of gac and florisil is 5‰ and 5‰ ...

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Abstract

The present invention discloses a strain of Bacillus cereus for decomposing proteins, wherein the Bacillus cereus is preserved in the China Center for Type Culture Collection, and has the preservation number of CGMCC NO:14049. The invention further discloses a method for preparing a soybean small peptide by using the Bacillus cereus, the obtained soybean small peptide, and a carbonic acid soda water health beverage. According to the present invention, the Bacillus cereus has strong protein decomposition ability, can be developed into the edible high-economic-added-value soybean small peptide and the related products thereof, and has significant economic value.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular, to a strain of Bacillus cereus (Bacillus scereus) used for decomposing proteins, a method for preparing soybean small peptides using the strain, and the obtained soybean small peptides. The present invention also relates to a carbonated soda healthy drink. Background technique [0002] Soybean is the most important and most productive bean in the world. As the most abundant and cheapest protein source that humans can eat, soybean contains comprehensive amino acids, especially lysine, which just complements the lysine in cereal protein. lack of. Asians like to eat soybean fermented products, including tempeh, soybean juice, soybean paste and various fermented bean curd products, which not only avoid the protein allergy symptoms that plague European and American people, but also eliminate the factors that inhibit nutrition through the action of microorganisms, and at the same ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23J3/34A23J3/16A23L2/38A23L2/52C12R1/085
CPCA23J3/16A23J3/346A23L2/38A23L2/52C12N1/20C12R2001/085C12N1/205
Inventor 王正全崔云云周依雯肖博超欧莹莹李元阳陈建康刘鹏扬刘恒
Owner SHANGHAI OCEAN UNIV
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