Method for manufacturing medical material, medical material, and anti-adhesion material
A manufacturing method and medical technology, applied in the field of manufacturing of medical materials, medical materials and anti-adhesion materials, can solve the problem of high water solubility and achieve high safety effect
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Embodiment 1
[0067] 1.0 g of sodium hyaluronate (molecular weight: 800,000 Da) powder was dispersed in 100 mL of 80% ethanol aqueous solution. After heating to 50° C. with stirring, 20 mL of acetic anhydride was added, followed by further heating and stirring for 1 hour. Centrifugation was performed, and the recovered precipitate was washed with ethanol and water, then dried and pulverized to obtain hyaluronic acid powder. 0.5 g of the obtained hyaluronic acid powder was dispersed in 50 mL of a 1% sodium hyaluronate (molecular weight: 800,000 Da) aqueous solution, flowed into a stainless steel dish with a length of 12 cm × a width of 10 cm, and dried in a constant temperature bath at 20° C. to obtain dry membrane. The obtained dry film was immersed in a treatment solution (20% acetic anhydride / 80% ethanol solution), and left at 50° C. for 1 hour to perform water insolubilization treatment to obtain a hyaluronic acid powder-hyaluronic acid composite film with a thickness of about 50 μm. T...
Embodiment 2
[0069] 50 mL of an aqueous solution of 1% sodium hyaluronate (molecular weight: 800,000 Da) was poured into a stainless steel tray measuring 12 cm long by 10 cm wide, and frozen in a -80° C. freezer. The frozen substance was subjected to vacuum freeze-drying (vacuum degree -20 Pa, shelf temperature 25° C.) to obtain a sponge formed of sodium hyaluronate. The obtained sponge was immersed in a treatment solution (20% acetic anhydride / 80% ethanol solution), and left at 50° C. for 1 hour to perform a water insolubilization treatment to obtain a hyaluronic acid sponge. The obtained sponge was pulverized using a shredder to obtain a crushed product (granular product) of hyaluronic acid. Disperse 0.5 g of the obtained crushed product in 50 mL of a 1% sodium hyaluronate (molecular weight: 800,000 Da) aqueous solution, pour it into a stainless steel tray with a length of 12 cm x a width of 10 cm, and dry it in a thermostat at 20° C. to obtain a dry film. The obtained dried film was im...
Embodiment 3
[0077] The composite membrane produced in Example 1 was immersed in a 10% by volume aqueous glycerin solution, air-dried, and placed in a sterilization bag. The anti-adhesion film with a thickness of about 50 μm was obtained by irradiating 25 kGy of radiation together with a sterilization bag for sterilization. Adult dogs (beagle, female, 1.5 years old, body weight about 10 kg) were treated with general anesthesia for laparotomy, and the epidermis of the ventral wall was peeled off in a square of 3 cm. Arrange the anti-adhesion film so as to cover the peeled part and close the abdomen. Two weeks later, a laparotomy was performed on the dog after treatment with general anesthesia, and no adhesions occurred. In addition, the anti-adhesion membrane placed (implanted) in the dog's body disappeared 2 weeks after the implantation. This is presumed to be because the carboxyl group of hyaluronic acid constituting the anti-adhesion film is gradually neutralized by sodium ions in the ...
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