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Method for preparing nutritious intestinal mucosa repair agent for animals by separating and extracting chick embryo EGF, as well as application of nutritious intestinal mucosa repair agent for animals

A technology of intestinal mucosa and repair agent, which is applied in the field of separation and extraction of chicken embryo EGF to prepare animal intestinal mucosa nutrition repair agent, which can solve the problems of high price, restriction of wide application, increase of material transport and metabolism in the body, and achieve the improvement of sucrase activity, Improve feed intake and growth performance, protect and promote the growth of mucosal epithelial cells

Active Publication Date: 2017-10-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

EGF has many biological effects, which can promote DNA, RNA and protein synthesis in cells; stimulate cell division, proliferation and differentiation in various tissues; inhibit gastric acid secretion, promote ulcer healing and epithelial tissue regeneration; increase material transport and metabolism in the body; The extracellular matrix and the synthesis of some substances (fruit acid, prostaglandin) in the body have the effect of regulating sperm, ovarian development and reproductive function. EGF is an important growth factor to promote the development of the stomach and intestinal tract of newborn animals, but the limited source of EGF, expensive The price restricts its wide application in the field of animal husbandry

Method used

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  • Method for preparing nutritious intestinal mucosa repair agent for animals by separating and extracting chick embryo EGF, as well as application of nutritious intestinal mucosa repair agent for animals
  • Method for preparing nutritious intestinal mucosa repair agent for animals by separating and extracting chick embryo EGF, as well as application of nutritious intestinal mucosa repair agent for animals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 100 15-day-old hatched healthy chicken embryos, shell them, weigh them, add 0.05mol / L acetic acid (4°C) at a ratio of 1:6 (g / g), homogenize at high speed for 30 minutes, and centrifuge at 1500 rpm After 20 minutes, take the supernatant; add 0.05 mol / L acetic acid (4°C) to the sediment to homogenize for 15 minutes, centrifuge at 1500 rpm for 20 minutes, take the supernatant, combine the two supernatants, and remove the supernatant For surface fat, add sodium benzoate (25g / L) to the supernatant, stir thoroughly to dissolve it, add 3mol / L NH 4 Adjust the pH to 6.0 with OH, stir for half an hour, let stand, filter under reduced pressure, take out the precipitate, dry the precipitate, add pre-cooled acetone (0.05g / ml acetone 4°C), stir to form a suspension, and stand at 4°C for 12h , a protein-like flocculent precipitate appeared, filtered under reduced pressure, washed the precipitate three times with acetone, and added zeolite powder (30 mesh) to the remaining precipi...

Embodiment 2

[0023] At the beginning of weaning of 25-day-old piglets, the control group was fed the control diet (see Table 1 for formula). The full-price feed of the test group was added with 200 grams of animal intestinal mucosa EGF nutritional repair agent per ton of the diet of the control group, and the above-mentioned feed was fed until 46 days old, and the growth performance of piglets was measured.

[0024] Table 1 Test diet composition and nutritional indicators

[0025]

[0026] The test results showed that compared with the control group, the average daily gain of the piglets in the test group increased by 31% (P<0.05), the feed-to-weight ratio decreased by 12% (P<0.05), and the diarrhea rate decreased by 85% (P<0.05). The villi height increased by 72% (P<0.05), the crypt depth decreased by 21% (P<0.05), the duodenal enterokinase activity increased by 58% (P<0.05), and the jejunum lactase activity increased by 39% (P<0.05). <0.05), the invertase activity increased by 42% (P...

Embodiment 3

[0028] 21-day-old healthy AA broilers, the control group was fed the control diet (see Table 2 for the formula), and the full-price feed of the test group was added with 100 grams of animal intestinal mucosa EGF nutritional repair agent per ton of the control group diet, and fed the above feed At the age of 35 days, the growth performance of broilers was measured.

[0029] Table 2 Test diet composition and nutritional indicators

[0030]

[0031] The test results showed that compared with the control group, the daily gain and feed intake of broilers in the test group increased by 9.17% (P<0.05) and 7.74% (P<0.05), respectively, and the length of duodenal villi increased by 14.20% (P<0.05). <0.05), duodenal villi length / crypt depth increased by 25.53% (P<0.01); ileal villi length / crypt depth increased by 13.63% (P<0.05), duodenal trypsin, amylase, fat Enzyme and total proteolytic activity increased by 44.61% (P<0.05), 98.34% (P<0.01), 36.47% (P<0.05) and 28.36% (P<0.05), re...

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Abstract

The invention discloses a method for preparing a nutritious intestinal mucosa repair agent for animals by separating and extracting chick embryo EGF, as well as an application of the nutritious intestinal mucosa repair agent for the animals. According to the method for preparing the nutritious intestinal mucosa repair agent for the animals, epidermal growth factors (EGF) are separated and extracted from rapidly growing and developing chick embryos; and the method specifically comprises the following steps of adjusting pH value of a homogenate solution so as to make EGF with positive charges, adsorbing the EGF by using water-insoluble benzoic acid so as to separate the EGF from the homogenate solution, and resolving the EGF from the benzoic acid by utilizing acetone which has a property of EGF insolubility so as to prepare a chicken-source-EGF-containing nutritious repair agent which is capable of promoting intestinal mucosa repair of the animals by adopting the principle design. The nutritious intestinal mucosa repair agent for the animals disclosed by the invention is added into full-value feeds for livestock and poultry at an addition amount of 0.05-0.3 kilograms / ton; and the added nutritious intestinal mucosa repair agent is capable of obviously improving structural functions of the intestinal mucosa of the animals, increasing feed intake and growth performance of the livestock and poultry, and significantly reducing diarrhea rate of weaned piglets.

Description

technical field [0001] The invention relates to a method for separating and extracting chicken embryo EGF to prepare an animal intestinal mucosa nutritional repair agent and its application. Background technique [0002] The animal intestine is not only a place for digestion and absorption of nutrients, but also an important immune organ. Maintaining normal intestinal function is crucial to animal growth and development. Under the conditions of nutrient deficiency, infection, stress and pathology, etc., animals can cause damage to intestinal structure and function, translocation of bacteria and endotoxin, and intestinal mucosal barrier dysfunction, etc., which seriously affect animal health and production performance. Production brings significant economic losses. Epidermal growth factor (EGF) is a member of the growth-promoting factor family and has a wide range of biological activities. It can promote the growth and development of gastrointestinal tissues, repair damaged ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/30A23K50/60A23K50/75A23K10/20A23K40/00
CPCA23K10/20A23K40/00A23K50/30A23K50/60A23K50/75
Inventor 卢建军董信阳姚静宜王友明胡彩虹
Owner ZHEJIANG UNIV
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