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Honey bee egg removal method for microinjection

A technology of microinjection and bee eggs, applied in animal husbandry and other directions, can solve problems such as the development of applications and basic research that hinders researchers from studying the special biological phenomena of bees and bees.

Active Publication Date: 2019-12-13
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

If this problem cannot be overcome, even the efficient and convenient Crispr / Cas9 technology cannot be applied in honey bees, and the desire to speed up the molecular screening of good traits of honey bees and the cultivation of new varieties with disease resistance and high yield through honey bee genome editing technology will also be shattered. this
It even hinders researchers from further studying the complex social behavior of bees and the basic research of special biological phenomena of bees

Method used

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  • Honey bee egg removal method for microinjection

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Effect test

Embodiment 1

[0025] Embodiment 1, a kind of honeybee egg removal method for microinjection, comprises the following steps:

[0026] (1) Restrict the queen bee for 24 hours with a needle-type king cage on the new honeycomb, and reward the bee colony at the same time, that is, feed honey in sufficient quantities;

[0027] (2) Transfer the needle-type queen cage of step (1) to other positions of the spleen, and limit the queen bee to lay eggs for 1 hour;

[0028] (3) Cut off the egg-laying area in the spleen under the needle king cage in step (2), use a needle to shovel off the beeswax with bee eggs under a stereomicroscope, and transfer it out of the nest room; pay attention during the transfer process Avoid bee eggs touching the walls of the nest;

[0029] (4) Take another nest foundation, press it into thin slices with a thickness of 1 mm, cut into a nest foundation strip with a width of 0.5 cm and a length of 6 cm, and use the stickiness of the new nest foundation strip to stick it on th...

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Abstract

The invention relates to a method of extracting bee eggs for micro-injection. The method comprises the steps that firstly, the biological characteristics of spawning of a queen bee are utilized to limit spawning of the queen bee; then, by utilizing the viscosity of beeswax and the characteristic that the bee eggs adhere to the bottom of a honeycomb in an upright mode, a grafting needle is adopted to shovel the beeswax at the bottoms of the bee eggs, and the beeswax is attached to comb foundation strips. Through the adoption of the method of extracting the bee eggs for the micro-injection, a large number of bee eggs extracted within one hour are transferred, the hatchability reach 94%, and the difficulty is solved that the hatchability of bee eggs within one hour is low. A large number of bee eggs extracted within one hour are transferred without damage, and method support is provided for the application of bee genome editing technologies in the field of bee breeding.

Description

technical field [0001] The invention relates to a method for removing bee eggs, in particular to a method for removing bee eggs for microinjection, and belongs to the technical field of bee breeding. Background technique [0002] Honey bee is an important economic insect, and its products and products are playing an increasingly important role in the health care industry and medical field. At the same time, bees are important pollinators of plants, and their pollination value and ecological role are immeasurable. However, as an important model organism, it lags far behind other model organisms in basic biological research and gene function research of bees. Analyzing the reasons, it can be found that the lack of effective gene editing tools in the field of honeybees is the main reason why its gene function research has long lagged behind other model insects. Although many researchers have explored methods suitable for honeybee genome editing operations, such as: sperm-medi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 苏松坤耿海洋聂红毅张猛
Owner FUJIAN AGRI & FORESTRY UNIV
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