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Method and special device for measuring behavior differences of different mouse crymodynia models

A mouse and behavioral technology, applied in diagnostic recording/measurement, application, medical science, etc., to achieve the effect of simple operation, low experimental cost, and easy operation

Inactive Publication Date: 2017-10-24
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: the purpose of the present invention is to overcome the defects of existing cold pain behavior measurement, to provide a new tool for cold pain measurement and its method for measuring the behavior difference of mouse pain model

Method used

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  • Method and special device for measuring behavior differences of different mouse crymodynia models
  • Method and special device for measuring behavior differences of different mouse crymodynia models
  • Method and special device for measuring behavior differences of different mouse crymodynia models

Examples

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Effect test

Embodiment 1

[0037] Such as figure 1 and figure 2 Shown, a kind of device of measuring cold pain behavior of mouse, it comprises box body 3, and the columnar tube 2 that is installed in box body 3, is positioned at the cover plate 1 of columnar tube 2 tops; Described cover plate 1 surface is provided with There are 14 small holes 4 with a diameter of 6 mm, and 4 small holes 4 with a diameter of 6 mm are provided at a height of 325 mm from the bottom of the cylindrical tube 2 to the box body. Put into the plexiglass plate 5 that has diameter 120mm, thickness 2mm in the cylindrical tube 2.

Embodiment 2

[0039] A method for measuring cold pain behavior of mice, said method comprising the following steps:

[0040] Step (1): first add ice to the box body 3 and the columnar tube 2, press it tightly, and put a plexiglass plate 5 with a diameter of 120 mm and a thickness of 2 mm in the columnar tube 2. During the experiment, open the cover plate 1 and put the mouse On the plexiglass plate 5, start the experiment. Using ice-cold plexiglass plate 5 to identify the cold pain behavior of different mice, the temperature change of the plexiglass plate is as follows: image 3 .

[0041] Step (2) adopting the cold plate instrument to identify the cold pain behavior of bone cancer pain mice includes the following steps:

[0042](2.1) Take mice (5-6 weeks, 20 in each group), adopt 1% ​​pentobarbital sodium 50mg / kg, after intraperitoneal injection anesthesia takes effect, fix in supine position, left hind limb shaved, and network Disinfect the skin with iodine, make a skin incision of abou...

Embodiment 3

[0053] A method for measuring cold pain behavior of mice, said method comprising the following steps:

[0054] Step (1): first add ice to the box body 3 and the columnar tube 2, press it tightly, and put a plexiglass plate 5 with a diameter of 120 mm and a thickness of 2 mm in the columnar tube 2. During the experiment, open the cover plate 1 and put the mouse On the plexiglass plate 5, start the experiment. Using ice-cold plexiglass plate 5 to identify the cold pain behavior of different mice, the temperature change of the plexiglass plate is as follows: figure 2 .

[0055] Step (2): Using a cold plate apparatus to identify the cold pain behavior of formaldehyde-induced pain mice.

[0056] (2.1) Select 6 male mice, put them in a container without ice, and adapt for 30 minutes. Ice was added to the container, and 5 minutes later, 10 ul of 1% formaldehyde solution was applied to the sole of the mouse, and the mouse was placed on a plexiglass plate on ice. 60 minutes of vid...

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Abstract

The invention discloses a method and special device for measuring the behavior differences of different mouse crymodynia models. The method includes the steps of firstly, manufacturing the device for measuring mouse crymodynia behaviors; secondly, building a mouse bone cancer pain model, a mouse neuropathic pain model and a mouse formalin induced pain model, and measuring the pain behavior differences of the different models. The device can accurately measure and reflect mouse crymodynia behavior differences. The method has the advantages that the researches of diseases related to crymodynia behaviors are benefited, the mouse crymodynia behavior differences can be reflected to the maximum degree, the quantitative measurement of the crymodynia sensitivity of various clinical diseases can be simulated favorably, the researches of pain occurrence mechanisms caused by various diseases and the target design of drugs for treating the diseases are benefited, and the method is promising in application prospect.

Description

technical field [0001] The invention relates to a method and a dedicated device for measuring behavioral differences of different mouse cold pain models. Background technique [0002] The research on pain treatment has always been the most challenging hot issue in the treatment of neurological diseases. Severe pain is often difficult to control and seriously affects the quality of life of patients. In the history of pain research in recent decades, scientists have been working to replicate animal models that better reflect the characteristics of human pain. These animal models partially reproduce the symptoms of human pain, hyperalgesia, and pain responses caused by light touch, pressure, etc. The successful replication of these animal models is the basis for our research on the mechanism of pain generation, and provides an effective tool for humans to understand the mechanism of pain generation and screen better therapeutic drugs. Here, the measurement and precise quantif...

Claims

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Application Information

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IPC IPC(8): A61B5/00
CPCA61B5/4824A61B2503/40A61B2503/42
Inventor 王长明顾乐盈阮永兰戴小斌于光唐宗湘
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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