Method of extracting bilirubin from bile and simultaneously co-producing cholesterol and lecithin

Abstract

The invention relates to a method of extracting bilirubin from bile and simultaneously co-producing cholesterol and lecithin. The method comprises the following steps: breaking a gallbladder to obtain bile, filtering bile to remove impurities; subjecting the bile to an alkalization treatment and a centrifugal treatment in sequence, collecting the filtrate, directly subjecting the filtrate to spray-drying to obtain dry powder, adding dry powder into a mixed solvent, carrying out extraction under a subcritical condition to obtain fat containing extract and degreased powder; adding fat containing extract into acetone, carrying out reflux extraction, collecting the filtrate and filter residues; evaporating and concentrating the filtrate, extracting cholesterol from concentrated filtrate, recovering the filter residues, extracting lecithin from filter residues; adding water and dichloromethane into degreased powder to form an extraction liquid, carrying out extraction to obtain suspension in the upper layer and a red liquid in the lower layer; extracting bilirubin from the red liquid, and extracting cholic acid from the suspension. The provided method can extract bilirubin, cholic acid, cholesterol and lecithin from bile at the same time, bile is comprehensively utilized, resources are saved, energy consumption is reduced, and sustainable development is realized.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for extracting bilirubin and bile acid from bile and co-producing cholesterol and lecithin. Background technique [0002] Bile is a special body fluid that flows in the biliary tract and is secreted by the mammalian liver. After being formed in the liver, it enters the gallbladder through the cystic duct and is stored there; it is released from the gallbladder after eating to digest the fat and lipids in the food. The vast majority of bile is water (about 97%), and there are many substances dissolved in water, including bile acids (about 2.5%) that can help fat digestion and absorption, and liver excretion bile that has nothing to do with digestion. Red pigment (about 0.4%). In addition, bile contains phospholipids, cholesterol, sodium, potassium, calcium, phosphate, carbonate, and a very small amount of protein. [0003] Cholic acid, also known as...

AI Technical Summary

Problems solved by technology

The following problems generally exist in the current process method: one is that the content of fatty acids, lecithin and cholesterol in the bile is relatively large, which has a great influence on the yield and purity of extracting bilirubin and cholic acid, and the recovery of lecithin and cholesterol is not considered. The second is that the purification of bilirubin mainly uses strong acidic conditions to extract with dichloromethane, which not only removes incomplete impurities, but also easily promotes the isomerization of bilirubin; the third is that the purification of cholic acid mainly uses repeated crystallization and activated carbon treatment, The process is cumbersome, there is a lot of loss, and there is a lack of effective measures to remove deoxycholic acid impurities

This shows that the yield and the purity of the extraction of bilirubin and cholic acid by the existing technological method are not very ideal

Images