A kind of enzymatic hydrolysis method of steaming material
A technology of enzymatic hydrolysis and steam explosion, applied in the direction of fermentation, etc., can solve the problems of reactor pollution, difficult cleaning, insufficient enzymatic hydrolysis, etc., and achieve the effect of easy pollution control, continuous operation and continuous discharge
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Embodiment 1
[0044] Embodiment 1 Enzymolysis method of the present invention
[0045] The enzymatic hydrolysis device for steaming material such as figure 1 shown.
[0046] First, corn stalks are steamed and exploded (the addition amount is 19500kg / h), deionized water (the addition amount is 6630kg / h), penicillin (the addition amount is 0.021kg / h) and 30% (v / v) NaOH solution (the addition amount is 323kg / h) into the premixer 1 from the premixer inlet A1, and the installation position of the pH meter 2 is at a certain distance from the premixer inlet A1 to ensure that the measured pH value is an accurate value for uniform material mixing. According to the test value of pH meter 2, by adjusting the mass flow rate of lye, the pH value of the premix is adjusted, and when the premix reaches the designated pH value 4.8, add cellulase (addition is 113kg / h) by enzyme port A2 ) mixed to prevent cellulase failure, to obtain a premix. The cellulase used is Novozymes' third generation cellulase...
Embodiment 2
[0051] The predetermined apparent viscosity value is 900 centipoise, and others refer to Example 1.
Embodiment 3
[0053] The predetermined apparent viscosity value is 1100 centipoise, and others refer to Example 1.
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