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A kind of enzymatic hydrolysis method of steaming material

A technology of enzymatic hydrolysis and steam explosion, applied in the direction of fermentation, etc., can solve the problems of reactor pollution, difficult cleaning, insufficient enzymatic hydrolysis, etc., and achieve the effect of easy pollution control, continuous operation and continuous discharge

Active Publication Date: 2021-07-09
STATE GRID CORP OF CHINA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, both batch and continuous enzymatic hydrolysis methods have certain defects.
The intermittent enzymatic hydrolysis method consumes a lot of stirring energy, and needs to feed in and out regularly, which is inconvenient to operate and cannot realize continuous production
The enzymatic hydrolysis process of the continuous enzymatic hydrolysis method is always carried out in the same reactor. When some steaming materials are contaminated, it is very easy to cause the entire reactor to be polluted in a large area during the advancement process, making it impossible to continue normal production, and the continuous reaction The volume of the device is large, once it is contaminated, it needs to be shut down for cleaning, and it is difficult to clean up, so the continuous enzymatic hydrolysis method has a greater risk
Moreover, the enzymolysis solution obtained by the batch and continuous enzymolysis methods contains a large amount of incomplete enzymolysis components, and the enzymolysis is not sufficient.

Method used

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  • A kind of enzymatic hydrolysis method of steaming material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 Enzymolysis method of the present invention

[0045] The enzymatic hydrolysis device for steaming material such as figure 1 shown.

[0046] First, corn stalks are steamed and exploded (the addition amount is 19500kg / h), deionized water (the addition amount is 6630kg / h), penicillin (the addition amount is 0.021kg / h) and 30% (v / v) NaOH solution (the addition amount is 323kg / h) into the premixer 1 from the premixer inlet A1, and the installation position of the pH meter 2 is at a certain distance from the premixer inlet A1 to ensure that the measured pH value is an accurate value for uniform material mixing. According to the test value of pH meter 2, by adjusting the mass flow rate of lye, the pH value of the premix is ​​adjusted, and when the premix reaches the designated pH value 4.8, add cellulase (addition is 113kg / h) by enzyme port A2 ) mixed to prevent cellulase failure, to obtain a premix. The cellulase used is Novozymes' third generation cellulase...

Embodiment 2

[0051] The predetermined apparent viscosity value is 900 centipoise, and others refer to Example 1.

Embodiment 3

[0053] The predetermined apparent viscosity value is 1100 centipoise, and others refer to Example 1.

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Abstract

The invention belongs to the field of biochemical engineering and relates to an enzymatic hydrolysis method of steamed explosives, which includes the following steps: carrying out an enzymatic hydrolysis reaction on the steamed explosives, and isolating a reaction mixture with an apparent viscosity of 350-1100 centipoise; The mixture continues to undergo enzymatic hydrolysis reaction. The enzymatic hydrolysis method is more complete, and the enzymatic hydrolysis method is divided into multi-stage processes, which is conducive to timely control and cleanup of possible pollution in each stage. The energy consumption of the enzymatic hydrolysis method is low, and the continuous operation of the overall process is achieved through the viscosity gradient formed by horizontal transportation and the sequential feeding and discharging of multiple reactors.

Description

Technical field [0001] The invention belongs to the field of biochemical engineering, and specifically relates to an enzymatic hydrolysis method for steaming explosive materials. Background technique [0002] At present, the enzymatic hydrolysis methods of steamed explosive materials include intermittent enzymatic hydrolysis methods and continuous enzymatic hydrolysis methods. The intermittent enzymatic hydrolysis method is to add the steamed explosive materials into the enzymatic hydrolysis tank in batches for stirring reaction. After the reaction of the previous batch of steamed explosive materials is completed, the enzymatic hydrolysis solution is discharged, and then the subsequent batch of steamed explosive materials is added for reaction. The continuous enzymatic hydrolysis method is to continuously pass the steamed explosive materials into the tubular reactor, and the materials are pushed forward in the reactor while reacting. The entire process is carried out in a cl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2203/00
Inventor 赵锦李双喜赵鹏翔张毅赵正凯卜令习杜婷赵承宇
Owner STATE GRID CORP OF CHINA