Rapid diagnosis method for rice bacterial fusicoccum sp

Abstract

The invention discloses a rapid diagnosis method for rice bacterial fusicoccum sp, and belongs to the technical field of plant diseases. The rice rapid diagnosis method comprises the following steps: a) investigating field symptoms; and b) carrying out molecular detection on a pathogen. Compared with the prior art, the rice rapid diagnosis method has the beneficial effects that a) when field symptoms are investigated, primary diagnosis is made according to typical symptoms of rice bacterial fusicoccum sp, that is, pale of early-stage hydropenia withering of rice grains, browning and rotting in middle and later periods, and brown streaks on leaf sheaths, and bacterial fusicoccum sp can be rapidly distinguished from other spike diseases; b) as a primer is designed according to gene sequences in only B.glumae, the rice rapid diagnosis method is good in PCR (Polymerase Chain Reaction) product specificity and high in detection result precision; c) a field sample lixivium is directly adopted as a template for PCR, the operation that DNA (Deoxyribonucleic Acid) is extracted after pathogenic bacteria are separated is avoided, and thus the diagnosis time is greatly shortened; d) the diagnosis method for the rice bacterial fusicoccum sp is simple, rapid and accurate.

Description

technical field [0001] The invention belongs to the technical field of plant disease diagnosis, and in particular relates to a rapid diagnosis method for rice bacterial ear blight. Background technique [0002] Bacterial panicle blight of rice (BPBR) is a newly rising worldwide important rice disease, and its pathogen is Burkholderia gliaculi ( Burkholderia glumae ), mainly harming the seedlings and ears of rice. The pathogens infect the seedlings, first water-soaked spots appear on the diseased leaves of the seedlings, and then turn brown, and the severely infected seedlings rot. Pathogens infect the grains, initially showing a water-deficient withered pale color, and then gradually turning yellowish brown or dark brown, and the glume color also changes, showing that the tips or bases of the inner and outer glumes are purple-brown, and the glumes are dark purple Brown; at the same time, there are brown streaks on the leaf sheath. [0003] BPBR was first discovered in Kyu...

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Problems solved by technology

[0006] Existing studies have shown that fungal ear blight, ear rot, and black powder of rice grains can also produce symptoms of grain browning and decay, which are similar to bacterial ear blight symptoms, and it is difficult to accurately diagnose only by field symptoms

In the experiment using PCR method to detect pathogenic bacteria, due to B. glutae and other Burkholderia B. gladioli , B. plantarii Isogenomic sequence homology is extremely high, and the designed primers are not specific enough to effectively distinguish these types of Burkholderia; in addition, when doing PCR testing, the pathogenic bacteria are generally isolated from the susceptible samples first, and then the pathogenic bacteria DNA is extracted Making templates for PCR reactions is time-consuming and labor-intensive

These have caused great interference for the rapid diagnosis of rice bacterial ear blight

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