Enrichment culture method of somatic embryos of pear trees

A technology of proliferation culture and proliferation medium, which is applied in the field of proliferation and culture of pear somatic embryos, can solve problems such as affecting the survival rate, serious viruses, and being susceptible to pests and diseases, and achieves the promotion of economic income, robust growth of seedlings, and guarantees. Effects of reproductive rate

Inactive Publication Date: 2017-11-10
ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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Problems solved by technology

[0002] Pear trees, belonging to the genus Pyrus in the family Rosaceae, are perennial deciduous fruit trees, widely distributed around the world, and have the advantages of adaptability, strong disease resistance, and long life. Fungal diseases. In spring and summer, it is vulnerable to pests and diseases. The virus is serious, and the use of inferior seedlings leads to the decline of the excellent characteristics of pear trees, such as affecting the survival rate, as well as the results and yield, which limits to a certain extent. economic income

Method used

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  • Enrichment culture method of somatic embryos of pear trees
  • Enrichment culture method of somatic embryos of pear trees
  • Enrichment culture method of somatic embryos of pear trees

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of proliferation culture process of pear tree somatic embryo is:

[0024] (1) Select embryos for cultivation: first select the young pear fruit within 35 days of pollination, take out the seeds after cutting, and peel off the embryo body;

[0025] (2) Differentiation culture: the embryo body obtained in step (1) was inoculated in the embryo differentiation medium, and cultured for 15 days at a temperature of 25-30° C., a light intensity of 1500-2000 lux, and a light time of 14 h / d. Obtain embryo differentiation seedlings, the differentiation medium includes 1 / 2 improved MS+0.2mgL -1 IAA+0.2mgL -1 BA+0.5gL -1 Agar+30gL -1 Sucrose, and the pH value is 5.9;

[0026] (3) Proliferation culture: transplant the embryonic differentiated seedling sections obtained in step (2) into the proliferation medium, and cultivate them under the conditions that the temperature is 25-30° C., the light intensity is 1800-2200 lux, and the light time is 14 h / d. On 15 days, new shoo...

Embodiment 2

[0029] A kind of proliferation culture process of pear tree somatic embryo is:

[0030] (1) Select embryos for cultivation: first select the young pear fruit within 46 days after pollination, take out the seeds after cutting, and peel off the embryo body;

[0031] (2) Differentiation culture: the embryo body obtained in step (1) was inoculated in the embryo differentiation medium, and cultured for 20 days at a temperature of 25-30° C., a light intensity of 1500-2000 lux, and a light time of 14 h / d. Obtain embryo differentiation seedlings, the differentiation medium includes 1 / 2 improved MS+0.4mgL -1 IAA+0.5mgL -1 BA+0.8gL -1 Agar+30gL -1 Sucrose, and the pH value is 6.4;

[0032] (3) Proliferation culture: transplant the embryonic differentiated seedling sections obtained in step (2) into the proliferation medium, and cultivate them under the conditions that the temperature is 25-30° C., the light intensity is 1800-2200 lux, and the light time is 14 h / d. On 20 days, new s...

Embodiment 3

[0035] A kind of proliferation culture process of pear tree somatic embryo is:

[0036] (1) select the embryo for cultivation: first select the pear tree fruit that is in the mature stage, take out the seed after cutting, and peel off the embryo body;

[0037] (2) Differentiation culture: the embryo body obtained in step (1) was inoculated in the embryo differentiation medium, and cultured for 18 days at a temperature of 25-30° C., a light intensity of 1500-2000 lux, and a light time of 14 h / d. Obtain embryo differentiation seedlings, the differentiation medium includes 1 / 2 improved MS+0.3mgL -1 IAA+0.4mg L -1 BA+0.8gL -1 Agar+30gL -1 Sucrose, and the pH value is 7.2;

[0038] (3) Proliferation culture: transplant the embryonic differentiated seedling sections obtained in step (2) into the proliferation medium, and cultivate them under the conditions that the temperature is 25-30° C., the light intensity is 1800-2200 lux, and the light time is 14 h / d. On the 18th day, new...

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Abstract

The invention discloses an enrichment culture method of somatic embryos of pear trees. The enrichment culture method comprises steps as follows: firstly, seeds are taken out after fruits of the pear tree are cut open, and embryo bodies are unhusked; then, an embryo differentiation culture medium is inoculated with the embryo bodies, the embryo bodies are cultured at the temperature of 25-30 DEG C and at the illumination intensity of 1,500-2,000 lux for 14 hours per day and cultured for 15-20 d, and embryo differentiated seedlings are obtained; finally, the embryo differentiated seedlings are transplanted into a proliferation medium, cultured at the temperature of 25-30 DEG C at the illumination intensity of 1,800-2,200 lux for 14 hours per day and cultured for 15-20 d and cultured for 15-20 d, new shoot seedlings are obtained, and the pear trees are formed through rooting, hardening and transplantation. By means of the enrichment culture method, the reproduction rate of the pear trees can be effectively guaranteed, so that the transplantation survival rate of the plants can reach 90% or above, the seedlings grow robustly, early fruiting of the pear trees can be guaranteed, the enrichment culture method has the characteristics of high yield and stable production, and increase of economic income is promoted.

Description

technical field [0001] The invention relates to a method for multiplying and culturing somatic embryos of pear trees, and belongs to the technical field of fruit tree cultivation. Background technique [0002] Pear trees, belonging to the genus Pyrus in the family Rosaceae, are perennial deciduous fruit trees. They are widely distributed around the world. They have the advantages of adaptability, strong disease resistance, and long life. However, in the traditional cultivation of pear trees, pear trees are susceptible to infection. Fungal diseases. In spring and summer, it is vulnerable to pests and diseases. The virus is serious, and the use of inferior seedlings has led to a decline in the excellent characteristics of pear trees, such as affecting the survival rate, as well as results and yields, which limits to a certain extent. economic income. Contents of the invention [0003] In order to solve the above problems, the object of the present invention is to provide a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 任俊鹏刘伟忠刘照亭毛妮妮刘亚柏郭建鲁群王志娟王翔
Owner ZHENJIANG WANSHAN HONGBIAN AGRI PARK
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